Eukaryotic ribosomes can recognize preproinsulin initiation codons irrespective of their position relative to the 5' end of mRNA.

Nature ·Vol. 299 ·No. 5880 ·1982-09-16 ·Pages 221-6

Lomedico PT, McAndrew SJ

Abstract

The functional assay of a eukaryotic mRNA, into which additional AUG codons have been introduced by in vitro mutagenesis, shows that a translational initiation site need not necessarily be the nearest AUG codon to the 5' end of a mRNA. Hence, the sequence surrounding the AUG, and not simply its position relative to the 5' end of mRNA, appears to be important in determining initiation efficiency.

MeSH Terms

Animals Base Sequence Cell-Free System Chromosome Deletion Codon Genetic Engineering Insulin Mutation Peptide Chain Initiation, Translational Proinsulin/genetics Protein Precursors/genetics RNA, Messenger/metabolism Rats Ribosomes/metabolism

Chemicals

Codon Insulin Protein Precursors RNA, Messenger preproinsulin Proinsulin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lomedico P T

McAndrew S J

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/299221a0

Published

1982-09-16

Pages

221-6

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/299221a0 View journal details

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Eukaryotic ribosomes can recognize preproinsulin initiation codons irrespective of their position relative to the 5' end of mRNA.

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