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PMID: 6294622 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Drosophila topoisomerase I: isolation, purification and characterization.

Nucleic acids research ·Vol. 10 ·No. 21 ·1982-11-11 ·Pages 6945-55

Javaherian K, Tse YC, Vega J

Abstract

We have purified and characterized topoisomerase I from Drosophila melanogaster. The molecular weight of the enzyme is 135,000; 100,000, 90,000, and 65,000 molecular weight products result from degradation of the enzyme. The enzyme relaxes both positive and negative supercoiled DNA. Mg++ is not absolutely required, but stimulates the enzymatic activity considerably.

MeSH Terms
Animals Cell Nucleus/enzymology DNA Topoisomerases, Type I/isolation & purification Drosophila melanogaster/enzymology Kinetics Magnesium/pharmacology Molecular Weight
Chemicals
DNA Topoisomerases, Type I Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Javaherian K
Tse Y C
Vega J
References (25)
25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.21.6945
Published
1982-11-11
Pages
6945-55
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326976
Subset
IM
Grants
NIGMS NIH HHS · GM24544 · United States
External Links
PubMed source DOI: 10.1093/nar/10.21.6945 PMC Full Text View journal details
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