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PMID: 211488 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification of a DNA nicking-closing enzyme from mouse L cells.

Nucleic acids research ·Vol. 5 ·No. 8 ·1978-08-00 ·Pages 2861-75

Tang D

Abstract

A DNA nicking-closing enzyme has been purified from the nuclei of mouse L cells to 90% homogeneity. The denatured and reduced form of the enzyme has a molecular weight of 68,000 which is in agreement with the molecular weight of the native enzyme as determined by gel filtration and by sucrose sedimentation velocity assuming the protein is globular. Therefore, the active form of the enzyme is a monopolypeptide. Its isoelectric point is pH 4.2 +/- 0.2. The nicking-closing activity does not require a cofactor and does not involve any sulfhydryl group. The enzyme requires 0.2 M NaCl and pH in the range of 6.5-7.5 for optimal activity.

MeSH Terms
Cell Nucleus/enzymology Chemical Precipitation Chromatography/methods DNA Topoisomerases, Type I/isolation & purification HeLa Cells/enzymology Histones/analysis Isoelectric Focusing Isoelectric Point L Cells/enzymology Molecular Weight
Chemicals
Histones DNA Topoisomerases, Type I
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Tang D
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.8.2861
Published
1978-08-00
Pages
2861-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342212
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.8.2861 PMC Full Text View journal details
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