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PMID: 6292868 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the fnr gene and primary structure of the Enr protein of Escherichia coli.

Nucleic acids research ·Vol. 10 ·No. 19 ·1982-10-11 ·Pages 6119-30

Shaw DJ, Guest JR

Abstract

The nucleotide sequence of a 1.64 kb fragment of E. coli DNA containing the fnr gene (regulatory gene for fumarate and nitrate reduction) was determined using the dideoxy chain termination method. The fnr coding region (750 bp) was identified, and the initiation and termination points of fnr transcription were located by RNA:DNA hybridisation with single-stranded M13 probes. The DNA fragment also contained the 5' end of a separately transcribed gene of unknown function. The deduced molecular weight (27947) of the Fnr protein was in agreement with that of the protein identified by the maxicell procedure, and the primary structure contained regions of homology with several transcriptional regulator proteins.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Composition Base Sequence DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Genes, Regulator Plasmids

Chemicals

Bacterial Proteins DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Shaw D J

Guest J R

References (29)

29 references, click to expand

The regulation of metabolism in facultative bacteria. 3. The effect of nitrate.
Biochim Biophys Acta. 1967 Oct 9;148(1):233-42 PMID: 4864932
Metabolic pathways for nitrate reduction in Escherichia coli.
Biochim Biophys Acta. 1968 Jul 16;162(1):39-48 PMID: 4385809
Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor.
J Gen Microbiol. 1976 Dec;97(2):145-60 PMID: 796407
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
The chromosomal location and pleiotropic effects of mutations of the nirA+ gene of Escherichia coli K12: the essential role of nirA+ in nitrite reduction and in other anaerobic redox reactions.
J Gen Microbiol. 1978 May;106(1):1-12 PMID: 206651
Laboratoire de Chimie Bactérienne C.N.R.S., Marsielle, France.
Mol Gen Genet. 1978 Apr 6;160(2):225-9 PMID: 349355
Sequence of the lacI gene.
Nature. 1978 Aug 24;274(5673):765-9 PMID: 355891
Simple method for identification of plasmid-coded proteins.
J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
A strategy of DNA sequencing employing computer programs.
Nucleic Acids Res. 1979 Jun 11;6(7):2601-10 PMID: 461197
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
Regulatory sequences involved in the promotion and termination of RNA transcription.
Annu Rev Genet. 1979;13:319-53 PMID: 94251
Recovery of DNA from gels.
Methods Enzymol. 1980;65(1):371-80 PMID: 7374460
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
A new computer method for the storage and manipulation of DNA gel reading data.
Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
Amplification of fumarate reductase synthesis with lambdafrdA transducing phages and orientation of frdA gene expression.
Mol Gen Genet. 1980;179(2):377-85 PMID: 6450867
Codon catalog usage is a genome strategy modulated for gene expressivity.
Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Molecular cloning of the fnr gene of Escherichia coli K12.
Mol Gen Genet. 1981;181(1):95-100 PMID: 6261088
Termination of transcription and its regulation in the tryptophan operon of E. coli.
Cell. 1981 Apr;24(1):10-23 PMID: 7016334
Translational initiation in prokaryotes.
Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
Operon fusions in the nitrate reductase operon and study of the control gene nir R in Escherichia coli.
Mol Gen Genet. 1981;182(3):477-9 PMID: 6457962
Codon preference and its use in identifying protein coding regions in long DNA sequences.
Nucleic Acids Res. 1982 Jan 11;10(1):141-56 PMID: 7063399
Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC).
Nucleic Acids Res. 1981 Dec 21;9(24):6827-40 PMID: 6278450
Cloning and sequence of the crp gene of Escherichia coli K 12.
Nucleic Acids Res. 1982 Feb 25;10(4):1363-78 PMID: 6280141
Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli.
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2427-31 PMID: 6283521
Amplification and product identification of the fnr gene of Escherichia coli.
J Gen Microbiol. 1982 Oct;128(10):2221-8 PMID: 6759613

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.19.6119

Published

1982-10-11

Pages

6119-30

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320955

Subset

Databases

GENBANK

J01608

External Links

PubMed source DOI: 10.1093/nar/10.19.6119 PMC Full Text View journal details

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Nucleotide sequence of the fnr gene and primary structure of the Enr protein of Escherichia coli.

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