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PMID: 349355 Published · ppublish English Journal Article

Laboratoire de Chimie Bactérienne C.N.R.S., Marsielle, France.

Molecular & general genetics : MGG ·Vol. 160 ·No. 2 ·1978-04-06 ·Pages 225-9

Chippaux M, Giudici D, Abou-Jaoudé A, Casse F, Pascal MC

Abstract

Mutants of E. coli, completely devoid of nitrite reductase activity with glucose or formate as donor were studied. Biochemical analysis indicates that they are simultaneously affected in nitrate reductase, nitrite reductase, fumarate reductase and hydrogenase activities as well as in cytochrome C552 biosynthesis. The use of an antiserum specific for nitrate reductase shows that the nitrate reductase protein is probably missing. A single mutation is responsible for this phenotype: the gene affected, nir R, is located close to tyr R i.e. at 29 min on the chromosomal map.

MeSH Terms
Escherichia coli/enzymology,genetics,metabolism Fumarates Genes Glucose/metabolism Mutation NADH, NADPH Oxidoreductases/genetics Nitrate Reductases/genetics Nitrite Reductases/genetics Oxidoreductases/genetics
Chemicals
Fumarates Oxidoreductases NADH, NADPH Oxidoreductases Nitrate Reductases Nitrite Reductases Glucose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chippaux M
Giudici D
Abou-Jaoudé A
Casse F
Pascal M C
References (22)
22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267485
Published
1978-04-06
Pages
225-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267485
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