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PMID: 6288663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antagonism of the B subunit of DNA gyrase eliminates plasmids pBR322 and pMG110 from Escherichia coli.

Journal of bacteriology ·Vol. 152 ·No. 1 ·1982-10-00 ·Pages 338-44

Wolfson JS, Hooper DC, Swartz MN, McHugh GL

Abstract

The constructed plasmid pBR322 and the native plasmid pMG110 were eliminated (cured) from growing Escherichia coli cells by the antagonism of the B subunit of the bacterial enzyme DNA gyrase. The antagonism may be by the growth of cells (i) at semipermissive temperatures in a bacterial mutant containing a thermolabile gyrase B subunit or (ii) at semipermissive concentrations of coumermycin A1, an antibiotic that specifically inhibits the B subunit of DNA gyrase. The kinetics of plasmid elimination indicate that plasmid loss occurs too rapidly to be explained solely by the faster growth of that plasmid-free bacteria and, therefore, represents interference with plasmid maintenance.

MeSH Terms
Aminocoumarins Coumarins/pharmacology Escherichia coli/enzymology,genetics,growth & development Mutation Plasmids Temperature Topoisomerase II Inhibitors
Chemicals
Aminocoumarins Coumarins Topoisomerase II Inhibitors coumermycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wolfson J S
Hooper D C
Swartz M N
McHugh G L
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50 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.1.338-344.1982
Published
1982-10-00
Pages
338-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221411
Subset
IM
Grants
NIAID NIH HHS · 5 T32 AI-07061-05 · United States
External Links
PubMed source DOI: 10.1128/jb.152.1.338-344.1982 PMC Full Text View journal details
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