Characterization of a plasmid mutation affecting maintenance, transfer and elimination by novobiocin.

Molecular & general genetics : MGG ·Vol. 174 ·No. 2 ·1979-07-13 ·Pages 127-33

Taylor DE, Levine JG

Abstract

We have isolated a mutant H group plasmid temperature-sensitive for plasmid maintenance. Unlike the wild type plasmid (pSD114), the mutant (pDT4) was eliminated at 37 degrees C and also at 30 degrees C after novobiocin treatment. The mutant plasmid interfered with host cell growth at the non-permissive temperature. Conjugative transfer of the mutant was reduced at 30 degrees C compared to the wild-type plasmid. Introduction of a coumermycin-novobiocin resistance DNA gyrase (cou) mutation into Escherichia coli prevented pDT4 elimination by novobiocin but did not affect the temperature-sensitive phenotype. The evidence indicates that the mutant plasmid used bacterial DNA gyrase for replication. Models to account for the behaviour of this unusual mutant are discussed.

MeSH Terms

DNA Topoisomerases, Type II/metabolism Escherichia coli/genetics Hot Temperature Mutation Novobiocin/pharmacology Phenotype Plasmids

Chemicals

Novobiocin DNA Topoisomerases, Type II

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Taylor D E

Levine J G

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29 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268350

Published

1979-07-13

Pages

127-33

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268350

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Characterization of a plasmid mutation affecting maintenance, transfer and elimination by novobiocin.

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