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PMID: 6281776 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cell surface properties of HLA antigens on Epstein-Barr virus-transformed cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 2 ·1982-01-00 ·Pages 608-12

Smith LM, Petty HR, Parham P, McConnell HM

Abstract

A number of monoclonal antibodies have been used to investigate the distributions and rates of lateral motion of the HLA-A,B, and-DR antigens on several Epstein--Barr virus-transformed B-cell lines. The lateral diffusion coefficients (D) of fluorescein conjugates of the monoclonal antibodies bound to the cell surface were determined by fluorescence recovery after pattern photobleaching. Ds of HLA-A and-B were found to be comparable and of the order of 10(-9) to 10(-10) cm2/sec for each of the seven monoclonal antibodies and four cell lines examined. The HLA antigens appear to be monomeric on the cell surface based on experiments using mixtures of arsanilic acid-conjugated and fluorescein-conjugated antibodies. Four monoclonal antibodies against DR antigens were examined. Two of these, Genox 3.53 and L243, labeled the cell surface uniformly and gave Ds comparable to those obtained for the HLA-A and -B antigens. The other two, DA2 and 2.06, rapidly patched on the cell surface and were immobile. The DA2, L243, and Genox 3.53 antibodies bound outside of the caps formed with the arsanilic acid-conjugated 2.06 antibody and a second-step rhodamine-conjugated rabbit anti-arsanilate antibody. This is consistent with recent biochemical evidence that there are multiple distinct antigens coded for by the HLA-DR region.

MeSH Terms
Antibodies, Monoclonal Cell Line Cell Transformation, Viral Diffusion HLA Antigens/analysis Herpesvirus 4, Human Humans Immunologic Capping Membrane Proteins/immunology
Chemicals
Antibodies, Monoclonal HLA Antigens Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith L M
Petty H R
Parham P
McConnell H M
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.2.608
Published
1982-01-00
Pages
608-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345795
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.2.608 PMC Full Text View journal details
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