Home LiteratureArticle Details
PMID: 233746 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Diffusion rates of cell surface antigens of mouse-human heterokaryons. I. Analysis of the population.

The Journal of cell biology ·Vol. 75 ·No. 2 Pt 1 ·1977-11-00 ·Pages 475-82

Edidin M, Wei TY

Abstract

The rate of appearance, in a newly formed heterokaryon population, of cells bearing completely intermixed mouse and human surface antigens may be used to estimate diffusion constants for antigens on individual cells. From this estimate, it appears that the surface antigens in most cells do not diffuse at the rate expected, but rather move more slowly, by a factor of ten or more, than expected from either measured or calculated diffusion constants for proteins freely mobile in the plane of a lipid membrane. Differences in diffusion rates between cells are not due to effects of Sendai virus, or of trypsin. Restrictions on diffusion are apparently not due to cytochalasin B- or Colcemid-sensitive elements.

MeSH Terms
Animals Antigens, Surface/analysis Cell Line Cell Membrane/drug effects,ultrastructure Cell Transformation, Viral Cytochalasin B/pharmacology Demecolcine/pharmacology Diffusion Humans Hybrid Cells/cytology Mice Parainfluenza Virus 1, Human/genetics
Chemicals
Antigens, Surface Cytochalasin B Demecolcine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Edidin M
Wei T Y
References (22)
22 references, click to expand
  1. EFFECT OF HALOGENATED PYRIMIDINES AND THYMIDINE ON GROWTH OF L-CELLS AND A SUBLINE LACKING THYMIDINE KINASE.
    Exp Cell Res. 1964 Jan;33:19-28 PMID: 14109133
  2. Morphological and virological investigation of human tissue cultures transformed with SV40.
    J Cell Comp Physiol. 1963 Apr;61:145-63 PMID: 13972152
  3. A survey of transplantable and transmissible animal tumors.
    J Natl Cancer Inst. 1953 Apr;13(5):1299-377 PMID: 13035452
  4. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons.
    J Cell Sci. 1970 Sep;7(2):319-35 PMID: 4098863
  5. Effect of ouabain and metabolic inhibitors on the Na and K movements and nucleotide contents of L cells.
    J Physiol. 1971 Mar;213(3):665-82 PMID: 5102532
  6. Rotational and translational diffusion in membranes.
    Annu Rev Biophys Bioeng. 1974;3(0):179-201 PMID: 4371655
  7. A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.
    Proc Natl Acad Sci U S A. 1969 Mar;62(3):852-9 PMID: 4308097
  8. Lateral diffusion of phodopsin in Necturus rods.
    Exp Eye Res. 1973 Dec 24;17(6):503-10 PMID: 4782837
  9. Lateral diffusion of rhodopsin in the photoreceptor membrane.
    Nature. 1974 Feb 15;247(5441):438-41 PMID: 4818543
  10. Subcellular separation and molecular nature of human histocompatibility antigens (HL-A).
    Nature. 1974 Feb 15;247(5441):457-61 PMID: 4131934
  11. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains.
    J Immunol. 1965 Aug;95(2):230-45 PMID: 4158328
  12. A form of reduced nicotinamide adenine dinucleotide-cytochrome b 5 reductase containing both the catalytic site and an additional hydrophobic membrane-binding segment.
    J Biol Chem. 1973 Feb 10;248(3):793-9 PMID: 4346350
  13. Modification of cell membranes with viral envelopes during fusion of cells with HVJ (Sendai virus).
    Exp Cell Res. 1975 Jul;93(2):368-78 PMID: 169133
  14. Biochemical properties and immunochemical-genetic relationships of mouse H-2 alloantigens.
    Biochim Biophys Acta. 1974 Apr 8;344(1):1-25 PMID: 4598853
  15. The effects of concanavalin A on the mobility of lymphocyte surface receptors.
    Exp Cell Res. 1973 Sep;81(1):143-55 PMID: 4543272
  16. Antigen cap formation in cultured fibroblasts: a reflection of membrane fluidity and of cell motility.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2456-9 PMID: 4560687
  17. Receptor mobility and receptor-cytoplasmic interactions in lymphocytes.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1442-6 PMID: 4514314
  18. Effects of phagocytosis and colchicine on the distribution of lectin-binding sites on cell surfaces.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):394-8 PMID: 4521810
  19. Mobility and diffusion in the plane of cell membrane.
    J Theor Biol. 1973 Jul;40(1):11-7 PMID: 4723546
  20. Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2409-13 PMID: 1065895
  21. Measurement of membrane protein lateral diffusion in single cells.
    Science. 1976 Feb 6;191(4226):466-8 PMID: 1246629
  22. Differences in density of Concanavalin A-binding sites due to differences in surface morphology of suspended normal and transformed 3T3 fibroblasts.
    J Cell Sci. 1975 Oct;19(1):21-32 PMID: 1176547
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.75.2.475
Published
1977-11-00
Pages
475-82
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109935
Subset
IM
Grants
NIADDK NIH HHS · AM 11202 · United States
NCI NIH HHS · CB 43922 · United States
External Links
PubMed source DOI: 10.1083/jcb.75.2.475 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2