Measurement of membrane protein lateral diffusion in single cells.

Science (New York, N.Y.) ·Vol. 191 ·No. 4226 ·1976-02-06 ·Pages 466-8

Edidin M, Zagyansky Y, Lardner TJ

Abstract

Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.

MeSH Terms

Cell Line Cell Membrane/metabolism Diffusion Fluorescent Dyes Lasers Proteins/metabolism

Chemicals

Fluorescent Dyes Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Edidin M

Zagyansky Y

Lardner T J

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.1246629

Published

1976-02-06

Pages

466-8

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.1246629 View journal details

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Measurement of membrane protein lateral diffusion in single cells.

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