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PMID: 315563 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Monoclonal antibody directed to a B-cell antigen present in rats, mice, and humans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4636-40

Gasser DL, Winters BA, Haas JB, McKearn TJ, Kennett RH

Abstract

Spleen cells from a LEW.AVN rat immunized with cells from an MNR rat were fused with mouse myeloma cells to produce hybrid cell lines. One of these hybridomas produced a monoclonal antibody that was cytotoxic for bone marrow-derived (B) but not thymus-derived (T) cells. The antigen defined by this antibody is determined by a gene linked to the major histocompatibility complex (MHC). The antigen is also present on B cells of most mouse strains and is determined by an MHC-linked gene in this species as well. In both rats and mice, the gene determining the antigen maps within the immune response region of the MHC. All human B-cell lines, but not T-cell lines, and B but not T cells of all human donors tested so far are also positive for this antigen. Among human-mouse somatic cell lines that have lost various human chromosomes, this B-cell antigen is present on all lines that are positive for HLA antigen but is absent from all lines that have lost HLA.

MeSH Terms
Animals Antibody Specificity Antigen-Antibody Reactions Antigens, Surface/analysis B-Lymphocytes/immunology Clone Cells/immunology Genes Humans Hybrid Cells/immunology Isoantibodies Isoantigens/analysis Major Histocompatibility Complex Mice Rats Species Specificity
Chemicals
Antigens, Surface Isoantibodies Isoantigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gasser D L
Winters B A
Haas J B
McKearn T J
Kennett R H
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4636
Published
1979-09-00
Pages
4636-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411634
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4636 PMC Full Text View journal details
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