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PMID: 6264152 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restriction and modification in Bacillus subtilis: gene coding for a BsuR-specific modification methyltransferase in the temperate bacteriophage phi 3T.

Journal of virology ·Vol. 38 ·No. 3 ·1981-06-00 ·Pages 1077-80

Noyer-Weidner M, Pawlek B, Jentsch S, Günthert U, Trautner TA

Abstract

The resistance of Phi3T DNA to degradation by the restriction enzyme BsuR or its isoschizomer HaeIII is due to obligatory modification of such DNA. Biochemical and genetical experiments indicate that Phi3T codes for a methyltransferase, which methylates Phi3T DNA itself or heterologous DNA at target sites 5'-GG(*)CC.

MeSH Terms

Bacillus subtilis Bacteriophages/genetics,metabolism DNA Restriction Enzymes/metabolism DNA, Viral/metabolism DNA-Cytosine Methylases Deoxyribonucleases, Type II Site-Specific Genes, Viral Methyltransferases/metabolism

Chemicals

DNA, Viral DNA modification methylase BsuR DNA-Cytosine Methylases Methyltransferases DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GGCC-specific type II deoxyribonucleases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Noyer-Weidner M

Pawlek B

Jentsch S

Günthert U

Trautner T A

References (17)

17 references, click to expand

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Restriction and modification in Bacillus subtilis. Localization of the methylated nucleotide in the BsuRI recognition sequence.
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Unusual base sequence arrangement in phage phi 29 DNA.
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Purification and properties of the Bsu endonuclease.
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Unusual behaviour of SPO1 DNA with respect to restriction and modification enzymes recognizing the sequence 5'-G-G-C-C.
Mol Gen Genet. 1980 Apr;178(1):229-31 PMID: 6247618
Restriction and modification in Bacillus subtilis: identification of a gene in the temperate phage SP beta coding for a BsuR specific modification methyltransferase.
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.38.3.1077-1080.1981

Published

1981-06-00

Pages

1077-80

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC171247

Subset

External Links

PubMed source DOI: 10.1128/JVI.38.3.1077-1080.1981 PMC Full Text View journal details

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Restriction and modification in Bacillus subtilis: gene coding for a BsuR-specific modification methyltransferase in the temperate bacteriophage phi 3T.

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