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PMID: 6261086 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2.

Molecular & general genetics : MGG ·Vol. 181 ·No. 1 ·1981-00-00 ·Pages 8-12

Stalker DM, Thomas CM, Helinski DR

Abstract

A DNA sequence consisting of 617 base pairs (bp) from the region of the origin of replication of the broad-host range plasmid RK2 has been determined. Included within this sequence is a 393 bp HpaII restriction fragment that provides a functional origin or replication when other essential RK2 specified functions are provided in trans. Also contained in this sequence is a region, distinguished functionally from the replication origin, which is involved in the expression of inc2 incompatibility, i.e., the ability of derivatives of RK2 to eliminate a resident RK2 plasmid. The 617 bp sequence includes eight 17 base pair direct repeats with 5 located within the region required for a functional replication origin and 3 within the region involved in inc2 incompatibility. In addition, a 40 bp region rich in A-T followed by a 60 bp stretch having a high G + C content is present. Deletion evidence indicates that the A-T rich and possibly the G + C regions are required for a functional replication origin. Based on the evidence contained in this and the preceding paper (Thomas et al. 1980 b) a model will be presented for the involvement of these specific sequences in the initiation of RK2 DNA replication, plasmid maintenance and plasmid incompatibility.

MeSH Terms
Base Sequence DNA Replication DNA Restriction Enzymes DNA, Bacterial/metabolism Escherichia coli/genetics Genes, Regulator R Factors Repetitive Sequences, Nucleic Acid Replicon
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stalker D M
Thomas C M
Helinski D R
References (29)
29 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00338997
Published
1981-00-00
Pages
8-12
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · 1-F32 GMO6542 · United States
NIAID NIH HHS · AI-07194 · United States
Databases
GENBANK
J01780
External Links
PubMed source DOI: 10.1007/BF00338997
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