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PMID: 277911 Published · ppublish English Journal Article

Site-specific initiation of a DNA fragment: nucleotide sequence of the bacteriophage G4 negative-strand initiation site.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 7 ·1978-07-00 ·Pages 3094-8

Sims J, Dressler D

Abstract

The synthesis of the bacteriophage G4 negative strand is an example of the de novo initiation of a polynucleotide chain. This initiation is performed by the Escherichia coli replication protein dna G which selects a unique site on 5400-base positive-strand template. In this paper we present the nucleotide sequence of the G4 negative-strand initiation site. This is the template element recognized by the dna G priming protein. In conjunction with the sequence of the nascent negative strand, obtained by Bouché, Rowen, and Kornberg [Bouché, J.-P., Rowen, L. & Kornberg, A. (1978) J. Biol. Chem. 253, 765-769], the present data provide a description of a dna G-dependent origin of replication in which one knows the place at which polymerization starts at the nucleotide level.

MeSH Terms
Base Sequence Chromosome Mapping Coliphages/genetics DNA Replication DNA Restriction Enzymes DNA, Single-Stranded/metabolism DNA, Viral/biosynthesis Genes, Viral Nucleic Acid Conformation Virus Replication
Chemicals
DNA, Single-Stranded DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sims J
Dressler D
References (24)
24 references, click to expand
  1. An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):645-9 PMID: 204927
  2. Migration of Escherichia coli dnaB protein on the template DNA strand as a mechanism in initiating DNA replication.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4190-4 PMID: 144914
  3. Site-specific initiation of a DNA fragment.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1652-6 PMID: 347442
  4. A ribo-deoxyribonucleotide primer synthesized by primase.
    J Biol Chem. 1978 Feb 10;253(3):770-4 PMID: 340459
  5. The RNA primer synthesized by primase to initiate phage G4 DNA replication.
    J Biol Chem. 1978 Feb 10;253(3):765-9 PMID: 340458
  6. Nucleotide sequences of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1081-5 PMID: 274698
  7. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  8. Physical structure of the replication origin of bacteriophage lambda.
    Science. 1977 Dec 9;198(4321):1051-6 PMID: 929187
  9. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  10. Nucleotide sequence of the intercistronic region between genes G and F in bacteriophage phiX174 DNA.
    J Mol Biol. 1976 Oct 15;107(1):1-24 PMID: 826639
  11. Conversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins.
    J Biol Chem. 1974 Jul 10;249(13):3999-4005 PMID: 4604274
  12. Multienzyme systems of DNA replication.
    Science. 1974 Dec 13;186(4168):987-93 PMID: 4620044
  13. Initiation of deoxyribonucleic acid synthesis. IV. Incorporation of the ribonucleic acid primer into the phage replicative form.
    J Biol Chem. 1973 Feb 25;248(4):1361-4 PMID: 4568814
  14. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  15. On the statistical significance of primary structural features found in DNA-protein interaction sites.
    Nucleic Acids Res. 1975 Mar;2(3):327-45 PMID: 1093137
  16. dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.
    J Biol Chem. 1975 Aug 10;250(15):5995-6001 PMID: 1097446
  17. Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4 PMID: 4610569
  18. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233-7 PMID: 4564209
  19. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9 PMID: 4554537
  20. Evolution of phi-chi 174. Isolation of four new phi-chi-like phages and comparison with phi-chi 174.
    Virology. 1974 Mar;58(1):272-89 PMID: 4821700
  21. A possible role for RNA polymerase in the initiation of M13 DNA synthesis.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9 PMID: 4941987
  22. Evolution of phi chi 174. II. A cleavage map of the G4 phage genome and comparison with the cleavage map of phi chi 174.
    Virology. 1975 Feb;63(2):320-5 PMID: 1078741
  23. Replication of phage G4. A novel and simple system for the initiation of deoxyribonucleic acid synthesis.
    J Biol Chem. 1975 Jun 25;250(12):4684-9 PMID: 1141224
  24. Statistical significance of DNA sequence symmetries.
    Nature. 1975 Jan 10;253(5487):128-30 PMID: 1110760
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.7.3094
Published
1978-07-00
Pages
3094-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392720
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.7.3094 PMC Full Text View journal details
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