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PMID: 375227 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 3 ·1979-03-00 ·Pages 1150-4

Stalker DM, Kolter R, Helinski DR

Abstract

A 2.1-kilobase segment of the antibiotic resistance plasmid R6K carries sufficient information to replicate as a plasmid in Escherichia coli. This segment contains a functional origin of replication and a structural gene for a protein, designated pi, that is required for the initiation of R6K replication. The nucleotide sequence of a 520-base-pair portion of this 2.1-kilobase segment that includes the functional origin of replication and the region adjacent to the start of the pi structural gene was determined. A striking feature of the sequence is the presence of seven 22-base-pair direct repeats joined in tandem in the region adjacent to the start of the pi gene. A possible role of the tandem repeats in the regulation of expression of the pi protein and the control of initiation of replication of the plasmid R6K is discussed.

MeSH Terms
Anti-Bacterial Agents/pharmacology Base Sequence Chromosome Mapping DNA Replication DNA Restriction Enzymes Drug Resistance, Microbial Escherichia coli/metabolism Genes Plasmids
Chemicals
Anti-Bacterial Agents DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stalker D M
Kolter R
Helinski D R
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.3.1150
Published
1979-03-00
Pages
1150-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383207
Subset
IM
Databases
GENBANK
M10713
External Links
PubMed source DOI: 10.1073/pnas.76.3.1150 PMC Full Text View journal details
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