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PMID: 6261081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of the replication control region of the resistance plasmid R1drd-19.

Molecular & general genetics : MGG ·Vol. 181 ·No. 1 ·1981-00-00 ·Pages 116-22

Stougaard P, Molin S, Nordström K, Hansen FG

Abstract

The region of plasmid R 1 containing the replication control genes has been sequenced using the Maxam-Gilbert method. The nucleotide sequence of two small PstI restriction fragments (a total of about 1,000 base pairs) was determined for the wild-type R 1 plasmid as well as for two different copy mutants. It was found that one copy mutant has a single base substitution in the fragment which was recently shown to harbor an important inc/cop gene (Molin and Nordström 1980). Furthermore, the sequence indicates the presence of a structural gene that codes for a polypeptide of size 10,500 daltons. Possible gene products predicted from the nucleotide sequences and their role in replication control are discussed.

MeSH Terms
Base Sequence DNA Replication DNA Restriction Enzymes Genes, Regulator R Factors
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stougaard P
Molin S
Nordström K
Hansen F G
References (31)
31 references, click to expand
  1. Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5.
    Mol Gen Genet. 1979 Jan 5;168(1):1-25 PMID: 372736
  2. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  3. Role of plasmid-coded RNA and ribonuclease III in plasmid DNA replication.
    Cell. 1979 Sep;18(1):61-71 PMID: 389434
  4. Molecular cloning of replication and incompatibility regions from the R-plasmid R6K.
    J Mol Biol. 1978 Sep 25;124(3):443-68 PMID: 361972
  5. Genetic analysis of the inter-relationship between plasmid replication and incompatibility.
    Mol Gen Genet. 1979 Jul 13;174(2):135-47 PMID: 386041
  6. Clustering of genes involved in replication, copy number control, incompatibility, and stable maintenance of the resistance plasmid R1drd-19.
    J Bacteriol. 1979 Apr;138(1):70-9 PMID: 374378
  7. Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.
    J Bacteriol. 1975 Nov;124(2):641-9 PMID: 1102525
  8. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  9. The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R1drd19.
    Mol Gen Genet. 1979 Mar 27;171(3):277-85 PMID: 377014
  10. Lambda transducing phages derived from a FinO- R100::lambda cointegrate plasmid: proteins encoded by the R100 replication/incompatibility region and the antibiotic resistance determinant.
    Mol Gen Genet. 1979 Nov;176(3):319-34 PMID: 160490
  11. The nucleotide sequence of the region surrounding the replication origin of an R100 resistance factor derivative.
    Mol Gen Genet. 1979 Mar 27;171(3):287-93 PMID: 286869
  12. Isolation and characterization of new copy mutants of plasmid R1, and identification of a polypeptide involved in copy number control.
    Mol Gen Genet. 1981;181(1):123-30 PMID: 6261082
  13. Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication.
    J Bacteriol. 1980 Jan;141(1):111-20 PMID: 6986353
  14. Runaway replication of plasmid R1 is not caused by loss of replication inhibitor activity of gene cop.
    J Bacteriol. 1980 Aug;143(2):1046-8 PMID: 7009544
  15. Structure of the orgin of DNA replication of bacteriophage fd.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):50-3 PMID: 272666
  16. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.
    J Mol Biol. 1968 Jul 14;34(2):331-46 PMID: 4938550
  17. Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.
    Mol Gen Genet. 1978 Jun 1;162(1):51-7 PMID: 353525
  18. Role of the tof gene in the production and perpetuation of the lambdadv plasmid.
    Mol Gen Genet. 1975 Dec 30;142(3):225-30 PMID: 1221305
  19. Restriction endonuclease mapping and mutagenesis of the F sex factor replication region.
    Mol Gen Genet. 1977 Apr 29;152(3):175-82 PMID: 327274
  20. Rapid CsCl gradients using a vertical rotor.
    Anal Biochem. 1979 Aug;97(1):196-20 PMID: 384845
  21. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  22. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1.
    J Bacteriol. 1979 Jan;137(1):92-104 PMID: 368043
  23. Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites.
    J Mol Biol. 1975 Dec 15;99(3):419-43 PMID: 765476
  24. Requirement of a plasmid-encoded protein for replication in vitro of plasmid R6K.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5381-5 PMID: 364478
  25. Low molecular weight RNA species encoded by a multiple drug resistance plasmid.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3673-7 PMID: 358192
  26. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.
    J Mol Biol. 1973 Apr 5;75(2):235-55 PMID: 4580676
  27. Analysis of a recessive plasmid copy number mutant: evidence for negative control of Col E1 replication.
    Cell. 1979 Oct;18(2):267-75 PMID: 387257
  28. Purification and properties of a DNA-binding protein with characteristics expected for the Cro protein of bacteriophage lambda, a repressor essential for lytic growth.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2249-53 PMID: 1065873
  29. A runaway-replication mutant of plasmid R1drd-19: temperature-dependent loss of copy number control.
    Mol Gen Genet. 1978 Oct 4;165(2):167-79 PMID: 366376
  30. Plasmid incompatibility: cloning analysis of an incFII determinant of R6-5.
    Nature. 1978 May 4;273(5657):27-32 PMID: 692666
  31. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00339014
Published
1981-00-00
Pages
116-22
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
J01772, V00326
External Links
PubMed source DOI: 10.1007/BF00339014
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