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PMID: 358192 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Low molecular weight RNA species encoded by a multiple drug resistance plasmid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 8 ·1978-08-00 ·Pages 3673-7

De Wilde M, Davies JE, Schmidt FJ

Abstract

Multiple drug resistance plasmid NR1 is shown to code for at least 10 low molecular weight RNAs. These species, ranging in size from 60 to 120 nucleotides, have been purified from minicells by two-dimensional gel electrophoresis and characterized by RNase T1 fingerprinting. Hybridization of purified RNAs to restriction endonuclease digests of NR1 DNA indicates that most are derived from the resistance transfer factor region of the plasmid genome. One RNA was found to be coded by the transposable tetracycline resistance element Tn10, and several are associated with DNA fragments that contain origins of replication.

MeSH Terms
Escherichia coli/genetics Genes Molecular Weight R Factors RNA/biosynthesis RNA, Bacterial/biosynthesis
Chemicals
RNA, Bacterial RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
De Wilde M
Davies J E
Schmidt F J
References (21)
21 references, click to expand
  1. Resistance transfer agents in Shigella.
    Biochem Biophys Res Commun. 1960 Dec;3:654-9 PMID: 13727669
  2. Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2710-4 PMID: 331313
  3. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  4. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis.
    J Biol Chem. 1973 Jul 25;248(14):5024-32 PMID: 4577761
  5. Size and base composition of RNA in supercoiled plasmid DNA.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3744-8 PMID: 4359488
  6. Structures of two glycyl-tRNAs from Staphylococcus epidermidis.
    Nat New Biol. 1972 May 10;237(71):44-5 PMID: 4503743
  7. New features of the regulation of the tryptophan operon.
    Science. 1975 Jul 4;189(4196):22-6 PMID: 1094538
  8. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.
    J Mol Biol. 1973 Apr 5;75(2):235-55 PMID: 4580676
  9. Conditionally lethal mutants of bacteriophage T4 defective in production of a transfer RNA.
    J Mol Biol. 1973 Dec 5;81(2):137-55 PMID: 4591184
  10. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  11. Eight transfer RNAs induced by infection of Escherichia coli with bacteriophage T4.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3703-7 PMID: 4566457
  12. Isolation and characterization of 2-methyladenosine from Escherichia coli tRNA Glu 2 , tRNA Asp 1 , tRNA His 1 and tRNA Arg .
    Biochim Biophys Acta. 1972 Feb 23;262(1):1-10 PMID: 4552900
  13. Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12.
    J Bacteriol. 1971 Jul;107(1):21-33 PMID: 4935321
  14. Specific hybridization of tyrosine transfer ribonucleic acids with DNA from a transducing bacteriophage phi-80 carrying the amber suppressor gene su 3.
    J Mol Biol. 1967 Nov 14;29(3):457-71 PMID: 4867493
  15. Location of non-DNA components of closed circular colicin E1 plasmid DNA.
    Nature. 1975 Feb 20;253(5493):652-4 PMID: 1089901
  16. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  17. Production, properties and utility of bacterial minicells.
    Curr Top Microbiol Immunol. 1975;69:1-84 PMID: 1098854
  18. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):64-8 PMID: 1108020
  19. EcoRI restriction endonuclease map of the composite R plasmid NR1.
    J Bacteriol. 1976 Jul;127(1):619-36 PMID: 776943
  20. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  21. The participation of sRNA in the enzymatic synthesis of O-L-lysyl phosphatidylgylcerol in Staphylococcus aureus.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):934-41 PMID: 5219701
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.8.3673
Published
1978-08-00
Pages
3673-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392848
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.8.3673 PMC Full Text View journal details
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