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PMID: 6261082 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of new copy mutants of plasmid R1, and identification of a polypeptide involved in copy number control.

Molecular & general genetics : MGG ·Vol. 181 ·No. 1 ·1981-00-00 ·Pages 123-30

Molin S, Stougaard P, Light J, Nordström M, Nordström K

Abstract

Site-specific deletions and insertions in the replication region of plasmid R1 have generated a new class of copy mutants that are present in the cell with 10-15-fold increased copy number. All mutations described inactivate a copy number control gene which is distinct from another cop inc gene that was identified previously (Molin and Nordström 1980). Insertion of the lac operon lacking the normal lac promoter has been used to determine the direction of transcription of this cop gene. The mutants may all be complemented by wild-type plasmid derivatives and are thus recessive. In incompatibility tests with wild-type R1 plasmids, these mutants are indistinguishable from the wild-type plasmid. It therefore seems that this cop function does not play an important role for the incompatibility function. A polypeptide, molecular weight 11,000, has been identified as being the product of this cop gene.

MeSH Terms
Bacterial Proteins/genetics Base Sequence DNA Replication DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific Genes, Regulator Mutation Peptides/genetics R Factors
Chemicals
Bacterial Proteins Peptides DNA Restriction Enzymes CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Molin S
Stougaard P
Light J
Nordström M
Nordström K
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00339015
Published
1981-00-00
Pages
123-30
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00339015
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