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PMID: 6260966 Published · ppublish English Journal Article

Viral DNA synthesized in vitro by avian retrovirus particles permeabilized with melittin. I. Kinetics of synthesis and size of minus- and plus-strand transcripts.

Journal of virology ·Vol. 37 ·No. 1 ·1981-01-00 ·Pages 109-16

Boone LR, Skalka AM

Abstract

We have examined the kinetics of synthesis of minus [(-)]- and plus [(+)]-strand viral DNA in melittin-permeabilized avian retrovirus particles. The reaction was biphasic. There was a very rapid initial rate, followed, after approximately 1 h, by a lower rate. Many discrete bands of subgenomic-length (-) strands were produced after 10 and 20 min of synthesis; genome-length (7.7-kilobase [kb]) (-) strands were detected within 30 min. Extension to an 8.0-kb (-)-strand species was evident by 60 min. This extension was inhibited by actinomycin D. Synthesis of (+) strands (which is also inhibited by actinomycin D) began early, before any (-) strands were completed, and continued for more than 4 h beyond the time when synthesis of full-length DNA had terminated. Two distinct species of (+)-strand DNA, 0.27 and 0.35 kb, could be observed at the earliest times. Their presence was quickly obscured by subsequent formation of (+)-strand molecules of molecular length between 0.2 and 2.0 kb.

MeSH Terms
Avian Leukosis Virus/drug effects,metabolism Avian Sarcoma Viruses/drug effects,metabolism Bee Venoms/pharmacology DNA, Viral/biosynthesis Dactinomycin/pharmacology Electrophoresis, Agar Gel Kinetics Melitten/pharmacology Molecular Weight
Chemicals
Bee Venoms DNA, Viral Dactinomycin Melitten
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boone L R
Skalka A M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.37.1.109-116.1981
Published
1981-01-00
Pages
109-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC170987
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.37.1.109-116.1981 PMC Full Text View journal details
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