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PMID: 163973 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virus.

Nature ·Vol. 253 ·No. 5492 ·1975-02-13 ·Pages 507-11

Guntaka RV, Mahy BW, Bishop JM, Varmus HE

Abstract

The DNA of avian sarcoma virus assumes a closed circular configuration before integration into the host cell chromosomal DNA. Ethidium bromide reduces the formation of superhelical viral DNA and concurrently blocks integration of the viral genome. Inhibition of integration of viral DNA results in the inhibition of virus replication.

MeSH Terms

Alpharetrovirus/metabolism Animals Cells, Cultured DNA, Circular/metabolism DNA, Viral/metabolism Deoxyribonucleases/metabolism Ducks Ethidium/pharmacology Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Viral/biosynthesis Tritium Uridine/metabolism Virus Replication/drug effects

Chemicals

DNA, Circular DNA, Viral RNA, Viral Tritium Deoxyribonucleases Ethidium Uridine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Guntaka R V

Mahy B W

Bishop J M

Varmus H E

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/253507a0

Published

1975-02-13

Pages

507-11

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/253507a0 View journal details

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Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virus.

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