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PMID: 6153806 Published · ppublish English Journal Article

Two species of full-length cDNA are synthesized in high yield by melittin-treated avian retrovirus particles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 2 ·1980-02-00 ·Pages 847-51

Boone LR, Skalka A

Abstract

A method of activating endogenous cDNA synthesis in avian retroviruses that results in the formation of two species of full-length cDNA in high yield is described. Tests of biological activity show infectivity of at least the same order of magnitude as for full-length cDNA made by other procedures. Melittin, the major component of bee venom, is used as an alternative to nonionic detergents to make the viral envelope permeable and thus activate the endogenous RNA-dependent DNA polymerase. This compound is a toxic peptide known to interact with phospholipid membranes. It appears to be less disruptive to the viral structure than detergents, resulting in a more efficient transcription of the viral genome. Preliminary tests indicate that this method will also prove useful for studying enzymatic activities associated with other enveloped viruses.

MeSH Terms
Alpharetrovirus/drug effects,genetics Bee Venoms/pharmacology DNA, Viral/biosynthesis Detergents/pharmacology Dose-Response Relationship, Drug Melitten/pharmacology Molecular Weight RNA-Directed DNA Polymerase/metabolism Virus Replication/drug effects
Chemicals
Bee Venoms DNA, Viral Detergents Melitten RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boone L R
Skalka A
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.2.847
Published
1980-02-00
Pages
847-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348378
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.2.847 PMC Full Text View journal details
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