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PMID: 6246488 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Supercoils in prokaryotic DNA restrained in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 3 ·1980-03-00 ·Pages 1331-5

Pettijohn DE, Pfenninger O

Abstract

Cells of Escherichia coli containing the plasmid F were gamma-irradiated with various doses to introduce determined numbers of single-strand breaks in the F DNA. The cells were then incubated to permit repair of the breaks while DNA gyrase was inhibited with coumermycin to limit restoration of any relaxed supercoil. Repaired, covalently continuous F DNA was isolated and its superhelical density was measured by two different methods. Both indicated that a major part (50-60%) of the negative superhelical turns were maintained in the repaired molecules, suggesting that the supercoils are restrained in vivo.

MeSH Terms
Aminocoumarins Coumarins/pharmacology DNA Repair/drug effects DNA, Bacterial/radiation effects DNA, Superhelical/radiation effects Escherichia coli F Factor Gamma Rays Nucleic Acid Conformation/radiation effects Pyrroles/pharmacology Topoisomerase II Inhibitors
Chemicals
Aminocoumarins Coumarins DNA, Bacterial DNA, Superhelical Pyrroles Topoisomerase II Inhibitors coumermycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pettijohn D E
Pfenninger O
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.3.1331
Published
1980-03-00
Pages
1331-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348488
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.3.1331 PMC Full Text View journal details
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