Home LiteratureArticle Details
PMID: 6215918 Published · ppublish English Journal Article

Purification and structural studies on the complement-system control protein beta 1H (Factor H).

The Biochemical journal ·Vol. 205 ·No. 2 ·1982-08-01 ·Pages 285-93

Sim RB, DiScipio RG

Abstract

An efficient procedure for the isolation of the complement-system control protein beta 1H (Factor H) from human plasma was developed. The chemical composition and physical characteristics of the protein were studied, and a sequence of 17 amino acid residues at the N-terminus was determined. Factor H is a single-polypeptide-chain glycoprotein of mol.wt. 155 000 containing 9.3% carbohydrate. Factor H is cleaved by plasma proteinases to a two-chain form. This cleavage can be mimicked by trypsin, and the two-chain form retains fully the C3b-inactivator cofactor activity of Factor H. The proteolytic fragments of Factor H are compared with those of other proteins (C4b-binding protein and erythrocyte C3b-receptor) that act as cofactors for C3b-inactivator.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Carbohydrates/analysis Chromatography, Gel Chromatography, Ion Exchange Complement C3b Inactivator Proteins/isolation & purification Complement Factor H Electrophoresis, Polyacrylamide Gel Humans Molecular Weight Peptide Fragments/analysis
Chemicals
Amino Acids CFH protein, human Carbohydrates Complement C3b Inactivator Proteins Peptide Fragments Complement Factor H
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sim R B
DiScipio R G
References (36)
36 references, click to expand
  1. Interaction of beta1H globulin with cell-bound C3b: quantitative analysis of binding and influence of alternative pathway components on binding.
    J Exp Med. 1978 Jun 1;147(6):1792-1805 PMID: 567241
  2. The role of C4-binding protein and beta 1H in proteolysis of C4b and C3b.
    J Exp Med. 1979 Aug 1;150(2):267-76 PMID: 458376
  3. An improved purification procedure for the third component of complement and beta 1H globulin from human serum.
    Mol Immunol. 1979 Oct;16(10):767-76 PMID: 118108
  4. Regulation of the amplification C3 convertase of human complement by an inhibitory protein isolated from human erythrocyte membrane.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5867-71 PMID: 293688
  5. Metabolism of human beta 1H: studies in man and experimental animals.
    Clin Exp Immunol. 1979 Dec;38(3):397-404 PMID: 535181
  6. The physiological breakdown of the third component of human complement.
    Mol Immunol. 1980 Jan;17(1):9-20 PMID: 7360115
  7. Identification of a major human serum DNA-binding protein as beta 1H of the alternative pathway of complement activation.
    Biochem Biophys Res Commun. 1980 May 14;94(1):61-7 PMID: 6248066
  8. High molecular weight complex in human plasma between vitamin K-dependent protein S and complement component C4b-binding protein.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2512-6 PMID: 6454142
  9. Activation of the alternative pathway of complement: efficient fluid-phase amplification by blockade of the regulatory complement protein beta1H through sulfated polyanions.
    Eur J Immunol. 1981 Apr;11(4):291-5 PMID: 6454580
  10. Complement receptor is an inhibitor of the complement cascade.
    J Exp Med. 1981 May 1;153(5):1138-50 PMID: 6910481
  11. The proteolytic activation systems of complement.
    Annu Rev Biochem. 1981;50:433-64 PMID: 7023363
  12. Pattern of degradation of human complement fragment, C3b.
    FEBS Lett. 1981 Sep 14;132(1):55-60 PMID: 6457754
  13. The covalent-binding reaction of complement component C3.
    Biochem J. 1981 Jan 1;193(1):115-27 PMID: 7305916
  14. Autolytic fragmentation of complement components C3 and C4 under denaturing conditions, a property shared with alpha 2-macroglobulin.
    Biochem J. 1981 Jan 1;193(1):129-41 PMID: 7305917
  15. Synthesis of two components of human complement, beta 1H and C3bINA, during fetal life.
    Acta Paediatr Scand. 1981 Sep;70(5):705-10 PMID: 6459707
  16. Binding of fluid-phase complement components C3 and C3b to human lymphocytes.
    Biochem J. 1981 Sep 15;198(3):509-18 PMID: 7326019
  17. Human C4-binding protein: N-terminal amino acid sequence analysis and limited proteolysis by trypsin.
    FEBS Lett. 1982 Jan 11;137(1):75-9 PMID: 7067825
  18. Purification of human C3b inactivator by monoclonal-antibody affinity chromatography.
    Biochem J. 1982 Apr 1;203(1):293-8 PMID: 7103942
  19. Factor D of the alternative pathway of human complement. Purification, alignment and N-terminal amino acid sequences of the major cyanogen bromide fragments, and localization of the serine residue at the active site.
    Biochem J. 1980 Jun 1;187(3):863-74 PMID: 6821372
  20. Ultraviolet absorption spectra of proteins and amino acids.
    Adv Protein Chem. 1952;7:319-86 PMID: 14933256
  21. The thiobarbituric acid assay of sialic acids.
    J Biol Chem. 1959 Aug;234(8):1971-5 PMID: 13672998
  22. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.
    Biochemistry. 1964 Mar;3:297-317 PMID: 14155091
  23. ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT.
    J Exp Med. 1965 Aug 1;122:277-98 PMID: 14316946
  24. The purification of human prothrombin.
    Thromb Diath Haemorrh. 1966 Dec 1;16(3):468-90 PMID: 5996669
  25. Measurement of protein concentration with interferences optics.
    Anal Biochem. 1969 Apr 4;28(1):216-21 PMID: 5781411
  26. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  27. Plasminogen: purification from human plasma by affinity chromatography.
    Science. 1970 Dec 4;170(3962):1095-6 PMID: 5475635
  28. The quantitation of glucosamine and galactosamine in glycoproteins after hydrolysis in p-toluenesulphonic acid.
    FEBS Lett. 1975 Dec 1;60(1):76-80 PMID: 1227961
  29. Modulation of C3b hemolytic activity by a plasma protein distinct from C3b inactivator.
    Science. 1976 Sep 10;193(4257):1011-3 PMID: 948757
  30. Control of the amplification convertase of complement by the plasma protein beta1H.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3268-72 PMID: 1067618
  31. The unactivated form of the first component of human complement, C1.
    Biochem J. 1976 Sep 1;157(3):541-8 PMID: 985398
  32. Proteolytic activation of protein C from bovine plasma.
    Biochemistry. 1976 Nov 2;15(22):4893-900 PMID: 990250
  33. Modulation of the alternative complement pathways by beta 1 H globulin.
    J Exp Med. 1976 Nov 2;144(5):1147-63 PMID: 62817
  34. Mechanism of action of the C3b inactivator: requirement for a high molecular weight cofactor (C3b-C4bINA cofactor) and production of a new C3b derivative (C3b').
    Immunochemistry. 1977 Nov-Dec;14(11-12):749-56 PMID: 146022
  35. Reaction mechanisms of beta1H globulin.
    Int Arch Allergy Appl Immunol. 1978;57(3):221-32 PMID: 77846
  36. Complement C3 convertase: cell surface restriction of beta1H control and generation of restriction on neuraminidase-treated cells.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2416-20 PMID: 276881
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2050285
Published
1982-08-01
Pages
285-93
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1158480
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2050285 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2