Home LiteratureArticle Details
PMID: 7326019 Published · ppublish English Journal Article

Binding of fluid-phase complement components C3 and C3b to human lymphocytes.

The Biochemical journal ·Vol. 198 ·No. 3 ·1981-09-15 ·Pages 509-18

Sim E, Sim RB

Abstract

It is known that a population of B-lymphocytes has receptors for the third component of complement, C3, and that these lymphocytes may be identified by their ability to form rosettes with sheep erythrocytes coated with covalently bound fragments of complement component C3. Human tonsil lymphocytes, enriched for B-cells, form rosettes with sheep erythrocytes coated with antibody and complement components C1, C4b and C3b (EAC143b cells). Fluid-phase C3 will inhibit rosette formation between EAC143b and human tonsil lymphocytes over the same concentration range as fluid-phase C3b. C3 is not cleaved to C3b during incubation with lymphocytes or with lymphocytes and EAC143b cells. Fluid-phase 125I-labelled C3 and 125I-labelled C3b bind to lymphocytes in a specific manner. The characteristics of binding of both radioiodinated C3 and radioiodinated C3b are very similar, but the binding oc C3 is again not a result of cleavage to C3b. Salicylhydroxamic acid does not inhibit binding of 125I-labelled C3 to tonsil lymphocytes at concentrations that completely inhibit binding of 125I-labelled C3 to EAC142 cells via the nascent binding site of C3b. It is concluded that C3 and C3b share a common feature involved in binding to lymphocytes bearing receptors for the third component of complement.

MeSH Terms
Animals Complement C3/immunology Complement C3b/immunology Complement C3c Electrophoresis, Polyacrylamide Gel Humans In Vitro Techniques Lymphocytes/immunology Receptors, Complement/immunology Rosette Formation Salicylamides/pharmacology
Chemicals
Complement C3 Receptors, Complement Salicylamides Complement C3b Complement C3c salicylhydroxamic acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sim E
Sim R B
References (37)
37 references, click to expand
  1. Immune hemolysis: a simplified method for the preparation of EAC'4 with guinea pig or with human complement.
    J Immunol. 1967 Aug;99(2):263-8 PMID: 4166243
  2. Lymphocyte binding of fluid phase mouse C3b.
    Immunology. 1979 Mar;36(3):461-70 PMID: 374253
  3. Evidence for an ester linkage between the labile binding site of C3b and receptive surfaces.
    J Immunol. 1979 Sep;123(3):1388-94 PMID: 38283
  4. Regulation of the amplification C3 convertase of human complement by an inhibitory protein isolated from human erythrocyte membrane.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5867-71 PMID: 293688
  5. Tissue C3b receptors.
    Clin Exp Immunol. 1979 Nov;38(2):316-22 PMID: 527266
  6. Receptor-mediated pinocytosis of mannose glycoconjugates by macrophages: characterization and evidence for receptor recycling.
    Cell. 1980 Jan;19(1):207-15 PMID: 6766809
  7. Complement receptors.
    Contemp Top Mol Immunol. 1977;6:145-76 PMID: 396104
  8. Activation of the complement system by antibody-antigen complexes: the classical pathway.
    Adv Protein Chem. 1979;33:1-71 PMID: 396780
  9. Identification of the membrane glycoprotein that is the C3b receptor of the human erythrocyte, polymorphonuclear leukocyte, B lymphocyte, and monocyte.
    J Exp Med. 1980 Jul 1;152(1):20-30 PMID: 6967510
  10. The kinetics of antibody binding to membrane antigens in solution and at the cell surface.
    Biochem J. 1980 Apr 1;187(1):1-20 PMID: 6967725
  11. Binding of soluble 125I-human C3b, the third component of complement, to specific receptors in human cultured B lymphoblastoids cells: characterization of a low affinity interaction.
    J Immunol. 1980 Sep;125(3):1332-9 PMID: 6967899
  12. Complement receptors on Raji cells. The presence of a new type of C3 receptor.
    Immunology. 1980 Sep;41(1):159-66 PMID: 7429548
  13. Selective suppression of retroviral gp70-anti-gp70 immune complex formation by prostaglandin E1 in murine systemic lupus erythematosus.
    J Exp Med. 1980 Dec 1;152(6):1645-58 PMID: 6969775
  14. Lateral distribution and diffusion of the C3b receptor of complement, HLA antigens, and lipid probes in peripheral blood leukocytes.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6587-91 PMID: 6935669
  15. The covalent-binding reaction of complement component C3.
    Biochem J. 1981 Jan 1;193(1):115-27 PMID: 7305916
  16. The separation of functionally distinct forms of the third component of human complement (C3).
    Biochem J. 1981 Mar 1;193(3):963-70 PMID: 6458280
  17. Treatment of human complement components C4 and C3 with amines or chaotropic ions. Evidence of a functional and structural change that provides uncleaved C4 and C3 with properties of their soluble activated froms, C4b and C3b.
    Scand J Immunol. 1981;13(5):413-31 PMID: 6171865
  18. Complement: purification of the first component from rabbit and guinea pig serum.
    Immunochemistry. 1968 May;5(3):311-4 PMID: 4174348
  19. Ultrastructural localization of the third and fourth components of complement on complement-cell complexes.
    Am J Pathol. 1968 Aug;53(2):253-61 PMID: 5667579
  20. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.
    J Exp Med. 1972 Aug 1;136(2):207-15 PMID: 4114496
  21. Two different complement receptors on human lymphocytes. One specific for C3b and one specific for C3b inactivator-cleaved C3b.
    J Exp Med. 1973 Oct 1;138(4):798-811 PMID: 4542735
  22. Complement-dependent B-cell activation by cobra venom factor and other mitogens?
    J Exp Med. 1974 Feb 1;139(2):337-54 PMID: 4589989
  23. Receptor for soluble C3 and C3b on human lymphoblastoid (RAJI) cells. Properties and biologocal significance.
    J Exp Med. 1974 Mar 1;139(3):696-711 PMID: 4591176
  24. Receptor for the fourth component of complement on human B lymphocytes and cultured human lymphoblastoid cells.
    J Exp Med. 1974 Nov 1;140(5):1336-47 PMID: 4547573
  25. Specificity of human lymphocyte complement receptors.
    J Exp Med. 1975 May 1;141(5):1163-80 PMID: 805205
  26. Separation and functional analysis of subpopulations of lymphocytes bearing complement and Fc receptors.
    Transplant Rev. 1975;25:98-120 PMID: 1103383
  27. The requirement for C3 receptors on the precursors of 19S and 7S antibody-forming cells.
    J Exp Med. 1976 May 1;143(5):1111-21 PMID: 944233
  28. Surface markers on human B and T lymphocytes. VIII. Association between complement and Epstein-Barr virus receptors on human lymphoid cells.
    Scand J Immunol. 1976;5(4):401-10 PMID: 59946
  29. Interaction of human lymphocytes with fluid phase human C3b detected by immunofluorescence.
    Eur J Immunol. 1976 Jan;6(1):52-6 PMID: 823035
  30. The role of membrane receptors for C3b and C3d in phagocytosis.
    J Exp Med. 1977 Feb 1;145(2):357-71 PMID: 833545
  31. The generation of memory cells. I. The role of C3 in the generation of B memory cells.
    Immunology. 1977 Jul;33(1):31-40 PMID: 301502
  32. Interaction between the third complement protein and cell surface macromolecules.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2701-5 PMID: 268620
  33. Complement bridges between cells analysis of a possible cell-cell interaction mechanism.
    J Exp Med. 1977 Dec 1;146(6):1484-99 PMID: 925610
  34. Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.
    Biochem Biophys Res Commun. 1978 Feb 28;80(4):849-57 PMID: 637870
  35. The purification and properties of the second component of human complement.
    Biochem J. 1978 Apr 1;171(1):99-107 PMID: 417728
  36. C3-mediated cytoadherence. II. Dependence of cell attachment on similar topographic distribution of the receptors (for C3) and the ligands (C3/C3b).
    J Immunol. 1978 Dec;121(6):2539-41 PMID: 102696
  37. Coated pits, coated vesicles, and receptor-mediated endocytosis.
    Nature. 1979 Jun 21;279(5715):679-85 PMID: 221835
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1980509
Published
1981-09-15
Pages
509-18
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163296
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1980509 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2