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PMID: 7103942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification of human C3b inactivator by monoclonal-antibody affinity chromatography.

The Biochemical journal ·Vol. 203 ·No. 1 ·1982-04-01 ·Pages 293-8

Hsiung L, Barclay AN, Brandon MR, Sim E, Porter RR

Abstract

Monoclonal antibody has been obtained to the human complement control protein C3b inactivator after immunization of mice with the enzyme prepared by conventional methods. Antibody from ascitic fluid was purified and coupled to Sepharose-CL-4B to give a specific affinity column, which was used to isolate C3b inactivator from human serum in 70% yield. The product was characterized by size, chain structure, amino acid analysis and proteolytic activity.

MeSH Terms
Amino Acids/analysis Animals Antibodies, Monoclonal Chromatography, Affinity/methods Complement C3b Inactivator Proteins/immunology,isolation & purification,metabolism Mice Mice, Inbred BALB C Mice, Inbred DBA Radioimmunoassay
Chemicals
Amino Acids Antibodies, Monoclonal Complement C3b Inactivator Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hsiung L
Barclay A N
Brandon M R
Sim E
Porter R R
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21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2030293
Published
1982-04-01
Pages
293-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1158222
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2030293 PMC Full Text View journal details
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