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PMID: 6211593 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of lambda receptor and beta-lactamase synthesis and export using cloned genes in a minicell system.

Molecular & general genetics : MGG ·Vol. 185 ·No. 2 ·1982-00-00 ·Pages 302-10

Clement JM, Perrin D, Hedgpeth J

Abstract

We have cloned lamB, the gene for lambda receptor (an outer membrane protein), on a small plasmid which also carries the gene for beta-lactamase (a periplasmic protein). We have identified a promoter in the region of malK, the gene immediately preceding lamB, which is active in minicells but relatively inactive in vitro. Using a minicell system, we have found that both lambda receptor and beta-lactamase are made as full length precursors which are subsequently processed. We also show that the lambda receptor precursor can be exported to the outer membrane before it is processed. Mature beta-lactamase is found only in the periplasm, suggesting that processing may be a requirement for export to the periplasm.

MeSH Terms
Bacteriophage lambda Biological Transport Cell Compartmentation Cloning, Molecular Escherichia coli/metabolism Gene Expression Regulation Membrane Proteins/metabolism Protein Precursors/metabolism Receptors, Virus/genetics,metabolism beta-Lactamases/genetics,metabolism
Chemicals
Membrane Proteins Protein Precursors Receptors, Virus beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clement J M
Perrin D
Hedgpeth J
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330802
Published
1982-00-00
Pages
302-10
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM27472 · United States
External Links
PubMed source DOI: 10.1007/BF00330802
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