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PMID: 4201774 Published · ppublish English Journal Article

Isolation of the bacteriophage lambda receptor from Escherichia coli.

Journal of bacteriology ·Vol. 116 ·No. 3 ·1973-12-00 ·Pages 1436-46

Randall-Hazelbauer L, Schwartz M

Abstract

A factor which inactivates the phage lambda can be extracted from Escherichia coli. This factor is a protein and is located in the outer membrane of the bacterial envelope. It is found in extracts of strains which are sensitive to phage lambda, but not in extracts of strains specifically resistant to this phage. We conclude that this factor is the lambda receptor, responsible for the specific adsorption of the phage lambda to E. coli cells. A partial purification of the lambda receptor is described. Inactivation of the phage by purified receptor is shown to be accompanied by the release of deoxyribonucleic acid from the phage.

MeSH Terms
Adsorption Bacterial Proteins/isolation & purification,pharmacology Carbon Radioisotopes Cell Wall/analysis Chemical Precipitation Chloroform Cholic Acids Chromatography Coliphages/drug effects DNA, Viral Edetic Acid Electrophoresis, Polyacrylamide Gel Escherichia coli/analysis,cytology Lysogeny Methanol Microscopy, Electron Mutation Receptors, Drug Tritium Ultracentrifugation
Chemicals
Bacterial Proteins Carbon Radioisotopes Cholic Acids DNA, Viral Receptors, Drug Tritium Chloroform Edetic Acid Methanol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Randall-Hazelbauer L
Schwartz M
References (20)
20 references, click to expand
  1. Location of the maltose A and B loci on the genetic map of Escherichia coli.
    J Bacteriol. 1966 Oct;92(4):1083-9 PMID: 5332865
  2. Damaging effects of ethylenediaminetetra-acetate and penicillins on permeability barriers in Gram-negative bacteria.
    Biochem J. 1966 Sep;100(3):675-82 PMID: 4961352
  3. Physical, chemical, and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate.
    J Biol Chem. 1968 Dec 25;243(24):6384-91 PMID: 4973230
  4. Genetic analysis of the maltose A region in Escherichia coli.
    J Bacteriol. 1969 May;98(2):559-67 PMID: 4891257
  5. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  6. Genetics of a chain-forming mutant of Escherichia coli. Transduction and dominance of the envA gene mediating increased penetration to some antibacterial agents.
    Genet Res. 1970 Aug;16(1):63-78 PMID: 4922970
  7. Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12.
    J Bacteriol. 1971 Mar;105(3):968-75 PMID: 4926688
  8. Cell-surface alterations in Escherichia coli K-12 with chromosmal mutations changing ampicillin resistance.
    Ann N Y Acad Sci. 1971 Jun 11;182:342-57 PMID: 4105640
  9. The nature of the colicin K receptor of Escherichia coli Cullen.
    J Exp Med. 1971 Mar 1;133(3):534-53 PMID: 4939151
  10. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.
    J Bacteriol. 1971 Oct;108(1):545-52 PMID: 4941569
  11. Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli.
    J Bacteriol. 1971 Oct;108(1):553-63 PMID: 5001205
  12. On some genetic aspects of phage lambda resistance in E. coli K12.
    Genetics. 1972 Jun;71(2):207-16 PMID: 4558324
  13. A mutant of Escherichia coli defective in DNA polymerase II activity.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3238-42 PMID: 4564210
  14. Purification and properties of the colicin E3 receptor of Escherichia coli.
    J Biol Chem. 1973 Mar 10;248(5):1797-806 PMID: 4348549
  15. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  16. Genetic study of a temperate bacteriophage of Escherichia coli. l. The genetic system of the bacteriophage.
    Ann Inst Pasteur (Paris). 1954 Dec;87(6):653-73 PMID: 14350339
  17. Bacterial viruses; with particular reference to adsorption/penetration.
    Annu Rev Microbiol. 1958;12:27-48 PMID: 13595600
  18. Band-centrifugation of macromolecules and viruses in self-generating density gradients.
    Proc Natl Acad Sci U S A. 1963 Jun;49:902-10 PMID: 13997382
  19. CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI.
    J Bacteriol. 1965 Jan;89:28-40 PMID: 14255678
  20. Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage lambda.
    Virology. 1956 Aug;2(4):565-74 PMID: 13352781
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.116.3.1436-1446.1973
Published
1973-12-00
Pages
1436-46
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246503
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.116.3.1436-1446.1973 PMC Full Text View journal details
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