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PMID: 6210374 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Processing of the large rRNA precursor: two proposed categories of RNA-RNA interactions in eukaryotes.

Journal of molecular evolution ·Vol. 20 ·No. 3-4 ·1984-00-00 ·Pages 362-7

Tague BW, Gerbi SA

Abstract

The 5.8S RNA gene of eukaryotes is separated from the 26-28S rRNA gene by the internal transcribed spacer 2 (ITS 2). A compilation of known ITS 2 sequences is presented here. Four characteristic features of the ITS 2 primary structure are shared by all vertebrates. In contrast, lower eukaryotes lack most of these features, suggesting that the excision of the ITS 2 transcript during processing may differ between vertebrates and lower eukaryotes. Since the transcripts of rRNA ITS 2 and mRNA introns share some similarity, analogies have been made between the mechanisms of their removal during RNA maturation. A model is proposed for hydrogen-bonding of U3 snRNA with the 5' end of the vertebrate ITS 2 transcript. This U3 snRNA-ITS 2 RNA interaction does not appear to be used in ITS 2 processing in lower eukaryotes. Instead, in lower eukaryotes a region within the ITS 2 itself has the potential to hydrogen-bond to the 5' end of the ITS 2 transcript.

MeSH Terms
Animals Base Sequence Fungi/genetics Invertebrates/genetics Models, Biological Nucleic Acid Conformation Nucleic Acid Precursors/genetics,metabolism RNA/metabolism RNA Precursors RNA Processing, Post-Transcriptional RNA, Ribosomal/biosynthesis,genetics,metabolism RNA, Small Nuclear Vertebrates/genetics
Chemicals
Nucleic Acid Precursors RNA Precursors RNA, Ribosomal RNA, Small Nuclear RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tague B W
Gerbi S A
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02104742
Published
1984-00-00
Pages
362-7
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Grants
NIGMS NIH HHS · PHS-GM 20261 · United States
External Links
PubMed source DOI: 10.1007/BF02104742 View journal details
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