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PMID: 6208454 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structural variants of the alpha-fetoprotein gene in different inbred strains of rat.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 153-8

Gal A, Nahon JL, Lucotte G, Sala-Trepat JM

Abstract

Two structural variants of the rat alpha-fetoprotein (AFP) gene have been detected in different inbred strains of rats by EcoRI or HindIII restriction enzyme cleavage of cellular DNA, agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat AFP cDNA probes. The type I AFP gene variant is characteristic of the Sprague-Dawley strain, and type II is found in Buffalo rats. These variants appear to represent two different allelic forms of the rat AFP gene since they are inherited in a normal Mendelian fashion when Sprague-Dawley and Buffalo rats are crossed. The mapping results suggest that the two allelic variants differ from each other by multiple cleavage site variations (base pair substitutions) and by an insertion or deletion of DNA sequences. An extensive sequence variation appears to exist between the two forms of the rat AFP gene; we have estimated that as much as 2.7% of the nucleotides in this region vary between the two alleles.

MeSH Terms
Animals DNA Restriction Enzymes Genes Genetic Variation Male Nucleic Acid Hybridization Rats Rats, Inbred BUF/genetics Rats, Inbred Lew/genetics Rats, Inbred Strains/genetics alpha-Fetoproteins/genetics
Chemicals
alpha-Fetoproteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gal A
Nahon J L
Lucotte G
Sala-Trepat J M
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332738
Published
1984-00-00
Pages
153-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332738
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