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PMID: 6176942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

No evidence for post-transcriptional control of albumin and alpha-fetoprotein gene expression in developing rat liver neoplasia.

Nucleic acids research ·Vol. 10 ·No. 6 ·1982-03-25 ·Pages 1895-911

Nahon JL, Gal A, Frain M, Sell S, Sala-Trepat JM

Abstract

Rot analysis of hybridization data using highly labeled alpha-fetoprotein (AFP) and albumin (32P)cDNA probes has been used to quantitate AFP and albumin mRNA sequences in RNA preparations from different subcellular fractions of developing rat liver and Morris hepatoma 7777. In addition, size analysis of these mRNA sequences has been carried out by electrophoretic fractionation on agarose gels containing methylmercury hydroxyde and hybridization to radioactive cloned albumin and AFP cDNA probes. In all the tissues examined (fetal, newborn and adult rat liver, and hepatoma 7777) most of the albumin and AFP mRNA sequences were found associated with the polysomes as mature mRNA molecules; less than 2% of these sequences were present in the nuclear or the non polysomal cytoplasmic compartments. The number of AFP mRNA molecules was found to decrease in parallel in all the cellular compartments during rat liver development. In Morris hepatoma 7777 the content of albumin mRNA was considerably decreased in all the cellular fractions as compared to normal liver. These results demonstrate that post-transcriptional control mechanisms leading to an accumulation of non-functional mRNA molecules are not implicated in the changes of expression of albumin and AFP genes during rat liver development and neoplasia.

MeSH Terms
Animals Animals, Newborn DNA/metabolism Genes Kinetics Liver/metabolism Liver Neoplasms, Experimental/metabolism Nucleic Acid Hybridization Polyribosomes/metabolism RNA, Messenger/genetics RNA, Ribosomal/genetics Rats Serum Albumin/genetics Subcellular Fractions/metabolism Transcription, Genetic alpha-Fetoproteins/genetics
Chemicals
RNA, Messenger RNA, Ribosomal Serum Albumin alpha-Fetoproteins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nahon J L
Gal A
Frain M
Sell S
Sala-Trepat J M
References (42)
42 references, click to expand
  1. A procedure for the isolation of enzymically active rat-liver nuclei.
    Biochem J. 1964 Aug;92(2):313-7 PMID: 4284460
  2. Sites of serum alpha-fetoprotein synthesis in the human and in the rat.
    J Clin Invest. 1967 Jun;46(6):1010-6 PMID: 6067376
  3. In vitro synthesis of the foetal alpha 1-globulin in man.
    Nature. 1969 Jun 28;222(5200):1296-9 PMID: 4182837
  4. The proption of the incorporation of leucine into albumin to that into totaprotein in rat liver and hepatoma Morris 5123 TC.
    Eur J Biochem. 1969 Sep;10(2):355-61 PMID: 4309870
  5. Synthesis of serum albumin, prealbumin, alpha-foetoprotein, alpha-1-antitrypsin and transferrin by the human yolk sac.
    Nature. 1970 Dec 5;228(5275):995-7 PMID: 5529798
  6. Early appearance of embryonic -globulin in rat serum during carcinogenesis with 4-dimethylaminoazobenzene.
    Cancer Res. 1971 Sep;31(9):1192-4 PMID: 4107187
  7. [Transitory cell antigens of rat liver. I. The secretion and synthesis of fetospecific serum proteins during hepatic development and regeneration].
    Int J Cancer. 1971 Jul 15;8(1):71-80 PMID: 5118211
  8. Alpha-fetoprotein in ontogenesis and its association with malignant tumors.
    Adv Cancer Res. 1971;14:295-358 PMID: 4107670
  9. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.
    Biochim Biophys Acta. 1972 Mar 14;262(2):220-6 PMID: 5017913
  10. Effect of estrogen on gene expression in the chick oviduct. II. Transcription of chick tritiated unique deoxyribonucleic acid as measured by hybridization in ribonucleic acid excess.
    Biochemistry. 1973 Jul 17;12(15):2809-16 PMID: 4719120
  11. Hepatocyte proliferation and alpha 1-fetoprotein in pregnant, neonatal, and partially hepatectomized rats.
    Cancer Res. 1974 Apr;34(4):865-71 PMID: 4814999
  12. Relationship of rat alpha 1-fetoprotein to growth rate and chromosome composition of Morris hepatomas.
    Cancer Res. 1974 Jun;34(6):1413-7 PMID: 4363658
  13. Alpha-fetoprotein as a marker of embryo-specific differentiations in normal and tumor tissues.
    Transplant Rev. 1974;20(0):3-37 PMID: 4135841
  14. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  15. A procedure for the quantitative recovery of homogeneous populations of undegraded free and bound polysomes from rat liver.
    Biochemistry. 1976 Apr 20;15(8):1704-12 PMID: 1268192
  16. Expression of an oncodevelopmental gene product (alpha-fetoprotein) during fetal development and adult oncogenesis.
    Cancer Res. 1976 Nov;36(11 Pt. 2):4239-49 PMID: 61804
  17. Quantitation of rat alpha-fetoprotein messenger RNA with a complementary DNA probe.
    J Biol Chem. 1977 Dec 10;252(23):8469-75 PMID: 72753
  18. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  19. Distribution of rat liver albumin mRNA membrane-bound and free in polyribosomes as determined by molecular hybridization.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5397-401 PMID: 271961
  20. Identification of a high molecular weight presumptive precursor to albumin mRNA in the nucleus of rat liver and hepatoma cell line H4AZC2.
    J Biol Chem. 1978 Mar 10;253(5):1328-31 PMID: 203590
  21. Isolation and characterization of poly(adenylic acid)-containing messenger ribonucleic acid from rat liver polysomes.
    Biochim Biophys Acta. 1978 Jun 22;519(1):173-93 PMID: 667061
  22. Summary: the molecular biology of the eukaryotic genome is coming of age.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1209-34 PMID: 209932
  23. Post-transcriptional suppression of globin gene expression in cells transformed by avian erythroblastosis virus.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3776-80 PMID: 211506
  24. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.
    Cell. 1978 Dec;15(4):1495-509 PMID: 103631
  25. alpha-Fetoprotein and albumin genes of rats: no evidence for amplification-deletion or rearrangement in rat liver carcinogenesis.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):695-9 PMID: 85303
  26. Distribution of RNA transcripts from structural and intervening sequences of the ovalbumin gene.
    Science. 1979 Apr 20;204(4390):314-6 PMID: 432646
  27. Changes in expression of albumin and alpha-fetoprotein genes during rat liver development and neoplasia.
    Biochemistry. 1979 May 29;18(11):2167-78 PMID: 87217
  28. alpha-Fetoprotein in cancer and fetal development.
    Adv Cancer Res. 1979;29:275-346 PMID: 89800
  29. Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data.
    Nucleic Acids Res. 1979 Aug 24;6(12):3911-21 PMID: 493129
  30. The rat serum albumin gene: analysis of cloned sequences.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60 PMID: 158758
  31. Effect of ammonia on the glutamate dehydrogenase catalyzed oxidative deamination of L-glutamate. The steady state.
    Biochemistry. 1979 Dec 25;18(26):5924-8 PMID: 518877
  32. Gel electrophoretic fractionation of RNAs by partial denaturation with methylmercuric hydroxide.
    Anal Biochem. 1979 Oct 15;99(1):200-6 PMID: 93855
  33. Control of albumin and alpha-fetoprotein expression in rat liver and in some transplantable hepatocellular carcinomas.
    Biochim Biophys Acta. 1979 Aug 29;564(1):173-8 PMID: 93970
  34. Molecular mechanisms of control of albumin and alphafetoprotein production: a system to study the early effects of chemical hepatocarcinogens.
    Cell Biol Int Rep. 1980 Mar;4(3):235-54 PMID: 6155219
  35. Changes in rat alpha 1-fetoprotein and albumin mRNA levels during fetal and neonatal development.
    J Biol Chem. 1980 Nov 10;255(21):10036-9 PMID: 6159351
  36. Developmental shifts in frequency distribution of polysomal mRNA and their posttranscriptional regulation in the sea urchin embryo.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4653-6 PMID: 6933514
  37. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  38. Post-transcriptional regulation of messenger abundance in rat liver and hepatoma.
    Nucleic Acids Res. 1980 Jul 25;8(14):3087-103 PMID: 6108552
  39. Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3521-5 PMID: 6167988
  40. Glucocorticosteroid suppression of alpha1-fetoprotein synthesis in developing rat liver. Evidence for selective gene repression at the transcriptional level.
    Biochemistry. 1981 Nov 10;20(23):6665-72 PMID: 6171301
  41. Fine structure and evolution of the rat serum albumin gene.
    Mol Cell Biol. 1981 Oct;1(10):871-83 PMID: 6287210
  42. Determination of nucleic acids in animal tissues.
    J Biol Chem. 1955 May;214(1):59-70 PMID: 14367363
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.6.1895
Published
1982-03-25
Pages
1895-911
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320579
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.6.1895 PMC Full Text View journal details
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