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PMID: 6203743 Published · ppublish English Journal Article

Transcription and DNA sequence of the BamHI L fragment of B95-8 Epstein-Barr virus.

The EMBO journal ·Vol. 3 ·No. 5 ·1984-05-00 ·Pages 1083-90

Biggin M, Farrell PJ, Barrell BG

Abstract

The sequence of the BamHI L fragment of B95-8 Epstein-Barr virus (EBV) DNA has been determined. The transcription starts of five promoters have been mapped to this fragment, using S1 mapping and either in vitro transcription or the primer extension technique. Dramatically increased levels of cytoplasmic poly(A) + RNAs, transcribed from these promoters, occur after treatment of B95-8 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA). For three of these promoters addition of phosphonoacetic acid (PAA) inhibits the effect of TPA, indicating that they give rise to late lytic cycle RNAs. The other two promoters give rise to early RNAs. Northern blot analysis indicates that one of the late promoters initiates two transcripts whose size differences are due to different splicing patterns. These two RNAs code for the 350/300 and 250/200 kd envelope glycoproteins of EBV. The sequences of these proteins would be of use in the production of a synthetic vaccine to prevent EBV infection.

MeSH Terms
Animals Base Sequence Callitrichinae Cell Line DNA Restriction Enzymes DNA, Viral/genetics Deoxyribonuclease BamHI Genes, Viral Herpesvirus 4, Human/genetics Lymphocytes Operon Poly A/genetics RNA/genetics RNA, Messenger Tetradecanoylphorbol Acetate/toxicity Transcription, Genetic/drug effects
Chemicals
DNA, Viral RNA, Messenger Poly A RNA DNA Restriction Enzymes Deoxyribonuclease BamHI Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Biggin M
Farrell P J
Barrell B G
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-05-00
Pages
1083-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557477
Subset
IM
Databases
GENBANK
V01509, V01554, V01555
External Links
PubMed source PMC Full Text View journal details
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