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PMID: 6196195 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A poliovirus type 1 neutralization epitope is located within amino acid residues 93 to 104 of viral capsid polypeptide VP1.

The EMBO journal ·Vol. 2 ·No. 11 ·1983-00-00 ·Pages 2019-24

Wychowski C, van der Werf S, Siffert O, Crainic R, Bruneau P, Girard M

Abstract

Using nuclease Bal31, deletions were generated within the poliovirus type 1 cDNA sequences, coding for capsid polypeptide VP1, within plasmid pCW119. The fusion proteins expressed in Escherichia coli by the deleted plasmids reacted with rabbit immune sera directed against poliovirus capsid polypeptide VP1 (alpha VP1 antibodies). They also reacted with a poliovirus type 1 neutralizing monoclonal antibody C3, but reactivity was lost when the deletion extended up to VP1 amino acids 90-104. Computer analysis of the protein revealed a high local density of hydrophilic amino acid residues in the region of VP1 amino acids 93-103. A peptide representing the sequence of this region was chemically synthesized. Once coupled to keyhole limpet hemocyanin, this peptide was specifically immunoprecipitated by C3 antibodies. The peptide also inhibited the neutralization of poliovirus type 1 by C3 antibodies. We thus conclude that the neutralization epitope recognized by C3 is located within the region of amino acids 93-104 of capsid polypeptide VP1.

MeSH Terms
Amino Acid Sequence Antigens, Viral/immunology Capsid/immunology Epitopes Peptide Fragments/chemical synthesis,immunology Poliovirus/immunology Viral Proteins/immunology Viral Structural Proteins
Chemicals
Antigens, Viral Epitopes Peptide Fragments Viral Proteins Viral Structural Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wychowski C
van der Werf S
Siffert O
Crainic R
Bruneau P
Girard M
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
2019-24
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555403
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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