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PMID: 6088796 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replicase gene of coxsackievirus B3.

Journal of virology ·Vol. 51 ·No. 3 ·1984-09-00 ·Pages 742-6

Stålhandske PO, Lindberg M, Pettersson U

Abstract

A cDNA copy covering two-thirds of the coxsackievirus B3 genome was cloned in the PstI site of the pBR322 vector. A nucleotide sequence containing the gene for the viral replicase and the 3' noncoding region of the coxsackievirus B3 genome was determined. The predicted amino acid sequence of the coxsackievirus B3 replicase was shown to be remarkably similar to that of the poliovirus 1 replicase. The 3' noncoding region, in contrast, was only weakly homologous to the poliovirus 1 sequence but showed a close relationship to the sequence of swine vesicular disease virus, a variant of coxsackievirus B5. A 13-nucleotide-long segment located near the polyadenylic acid junction is conserved in several members of the enterovirus group and may thus serve an important function during replication of viral RNA.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes Enterovirus B, Human/enzymology,genetics Genes Genes, Viral HeLa Cells/enzymology Humans RNA Nucleotidyltransferases/genetics RNA-Dependent RNA Polymerase/genetics
Chemicals
RNA Nucleotidyltransferases RNA-Dependent RNA Polymerase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stålhandske P O
Lindberg M
Pettersson U
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.51.3.742-746.1984
Published
1984-09-00
Pages
742-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255839
Subset
IM
Databases
GENBANK
K02709
External Links
PubMed source DOI: 10.1128/JVI.51.3.742-746.1984 PMC Full Text View journal details
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