Home LiteratureArticle Details
PMID: 6325492 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of enteroviruses by spot hybridization.

Journal of clinical microbiology ·Vol. 19 ·No. 3 ·1984-03-00 ·Pages 436-8

Hyypiä T, Stålhandske P, Vainionpää R, Pettersson U

Abstract

A cloned partial cDNA copy of the coxsackievirus B3 genome was used for detecting enteroviruses in infected cells by employing a nucleic acid hybridization procedure. Cells infected with coxsackieviruses A and B, echovirus, and poliovirus gave positive hybridization signals, whereas cells infected with nonrelated viruses did not.

MeSH Terms
Base Sequence Enterovirus/isolation & purification Feces/microbiology Humans Nucleic Acid Hybridization RNA, Viral/analysis
Chemicals
RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hyypiä T
Stålhandske P
Vainionpää R
Pettersson U
References (9)
9 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. A dot hybridisation assay for detection of rotavirus.
    Lancet. 1983 Mar 12;1(8324):555-8 PMID: 6131257
  3. Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic Acid hybridization.
    Science. 1981 Aug 7;213(4508):670-2 PMID: 17847478
  4. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  5. An assay for the detection of the DNA genome of hepatitis B virus in serum.
    J Med Virol. 1982;9(1):57-68 PMID: 7061996
  6. Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6851-5 PMID: 6256764
  7. Identification of DNA viruses by membrane filter hybridization.
    J Clin Microbiol. 1982 Apr;15(4):744-7 PMID: 6279697
  8. Novel test for rapid viral diagnosis: detection of adenovirus in nasopharyngeal mucus aspirates by means of nucleic-acid sandwich hybridisation.
    Lancet. 1983 Feb 19;1(8321):381-3 PMID: 6130378
  9. Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.
    N Engl J Med. 1983 Apr 21;308(16):921-5 PMID: 6300675
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.19.3.436-438.1984
Published
1984-03-00
Pages
436-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC271084
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.19.3.436-438.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2