Home LiteratureArticle Details

PMID: 6279697 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of DNA viruses by membrane filter hybridization.

Journal of clinical microbiology ·Vol. 15 ·No. 4 ·1982-04-00 ·Pages 744-7

Stålhandske P, Pettersson U

Abstract

The use of membrane filter hybridization for the identification of DNA viruses is described. We designed and used a procedure for identification of herpes simplex virus. This method can discriminate between herpes simplex virus types 1 and 2 in a simple way.

MeSH Terms

Animals Cell Line Chlorocebus aethiops DNA Viruses/classification DNA, Viral/biosynthesis Kinetics Methods Nucleic Acid Hybridization Simplexvirus/classification,metabolism

Chemicals

DNA, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stålhandske P

Pettersson U

References (10)

10 references, click to expand

A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
Genetic relatedness of type 1 and type 2 herpes simplex viruses.
J Virol. 1972 May;9(5):738-45 PMID: 4337161
Studies on the relatedness of herpesviruses through DNA-DNA hybridization.
Virology. 1972 Jul;49(1):95-101 PMID: 4338997
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6851-5 PMID: 6256764
Optimized assay for thymidine kinase and its application to the detection of antibodies against herpes simplex virus type 1- and 2-induced thymidine kinase.
Infect Immun. 1980 Aug;29(2):425-34 PMID: 6260651
Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid:deoxyribonucleic acid hybridization.
J Clin Microbiol. 1980 Aug;12(2):226-34 PMID: 6262368
A rapid boiling method for the preparation of bacterial plasmids.
Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.15.4.744-747.1982

Published

1982-04-00

Pages

744-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC272179

Subset

External Links

PubMed source DOI: 10.1128/jcm.15.4.744-747.1982 PMC Full Text View journal details

Analysis Services

Identification of DNA viruses by membrane filter hybridization.

Contact

WeChat Official Account