Recombination after protoplast fusion in the yeast Candida tropicalis.

Archives of microbiology ·Vol. 115 ·No. 2 ·1977-11-18 ·Pages 143-9

Fournier P, Provost A, Bourguignon C, Heslot H

Abstract

Candida tropicalis protoplasts obtained by snail enzyme treatment were induced to fuse by the use of polyethylene-glycol. Heterokaryons formed by two auxotrophic strains were selected by complementation on minimal medium. These heterokaryons were unstable and readily dissociated into their nuclear components. Under appropriate conditions, the parental nuclei of an heterokaryon fused. The homokaryon so obtained was unstable and segregated into various types of auxotrophic and prototrophic recombinants.

MeSH Terms

Candida/drug effects,genetics,ultrastructure Cell Nucleus Drug Resistance, Microbial Flucytosine/pharmacology Heterozygote Polyethylene Glycols/pharmacology Protoplasts/metabolism,ultrastructure Recombination, Genetic

Chemicals

Polyethylene Glycols Flucytosine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Fournier P

Provost A

Bourguignon C

Heslot H

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17 references, click to expand

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Article Info

Journal

Archives of microbiology

Abbr.

Arch Microbiol

ISSN

0302-8933

DOI

10.1007/BF00406367

Published

1977-11-18

Pages

143-9

Language

English

Region

Germany

NLM ID

0410427

Subset

External Links

PubMed source DOI: 10.1007/BF00406367 View journal details

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Recombination after protoplast fusion in the yeast Candida tropicalis.

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