Protoplasts of Schizosaccharomyces pombe: an improved method for their preparation and the study of their guanine uptake.

Journal of general microbiology ·Vol. 90 ·No. 2 ·1975-10-00 ·Pages 260-4

Housset P, Nagy M, Schwencke J

Abstract

A new method is described for the efficient conversion of Schizosaccharomyces pombe cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: Km value, requirement for an energy source, sensitivity to competitive inhibitors, pH optimum, as well as the typical variation of the initial velocity of uptake observed during the growth phase.

MeSH Terms

Adenine/pharmacology Ascomycota/metabolism Azaguanine/pharmacology Azides/pharmacology Cell Wall/metabolism Culture Media Glycoside Hydrolases/metabolism Growth Guanine/metabolism Hydrogen-Ion Concentration Hypoxanthines/pharmacology Polysaccharides Protoplasts/metabolism,physiology Saccharomyces/growth & development Schizosaccharomyces/metabolism Snails/enzymology

Chemicals

Azides Culture Media Hypoxanthines Polysaccharides Guanine Glycoside Hydrolases Adenine Azaguanine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Housset P

Nagy M

Schwencke J

Article Info

Journal

Journal of general microbiology

Abbr.

J Gen Microbiol

ISSN

0022-1287

DOI

10.1099/00221287-90-2-260

Published

1975-10-00

Pages

260-4

Language

English

Region

England

NLM ID

0375371

Subset

External Links

PubMed source DOI: 10.1099/00221287-90-2-260

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Protoplasts of Schizosaccharomyces pombe: an improved method for their preparation and the study of their guanine uptake.

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