Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions.

Archives of microbiology ·Vol. 105 ·No. 3 ·1975-11-07 ·Pages 201-5

Anné J, Peberdy JF

Abstract

Solutions containing polyethylene glycol MW 6000 (PEG) induced fusion of protoplasts of Penicillium chrysogenum. Balanced heterokaryons were formed by fusion of nutritionally complementing protoplasts. Heterokaryotic fusion products were obtained up to a frequency of 4% of the number of protoplasts, surviving the fusion treatment. Investigation of the conditions, necessary to achieve this high fusion frequency, showed that supplementing the PEG solution with Ca++ and adjustment to high pH gave the best results. Mechanisms of fusion of fungal protoplasts by PEG, calcium and alkaline pH are discussed in view of the obtained results.

MeSH Terms

Calcium/pharmacology Cell Aggregation/drug effects Cell Fusion/drug effects Hydrogen-Ion Concentration Magnesium/pharmacology Penicillium/cytology Penicillium chrysogenum/cytology Polyethylene Glycols/pharmacology Protoplasts Temperature

Chemicals

Polyethylene Glycols Magnesium Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Anné J

Peberdy J F

References (11)

11 references, click to expand

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Article Info

Journal

Archives of microbiology

Abbr.

Arch Microbiol

ISSN

0302-8933

DOI

10.1007/BF00447138

Published

1975-11-07

Pages

201-5

Language

English

Region

Germany

NLM ID

0410427

Subset

External Links

PubMed source DOI: 10.1007/BF00447138 View journal details

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Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions.

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