Protoplasts obtained from Candida tropicalis grown on alkanes.
A method for the preparation of protoplasts from Candida tropicalis cultivated on n-tetradecane is described. This essentially consists of replacing the mannitol-sorbitol solution of the classical helicase technique by 1 m magnesium sulfate and lowering the pH to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming oxygen in the presence of n-tetradecane, n-decane, 1-decanol, and glucose.
6 references, click to expand
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Loss of the ability to oxidize hydrocarbons in protoplasts of Candida lipolytica.Experientia. 1967 Dec 15;23(12):1005-6 PMID: 6077860
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Formation of aberrant cell walls and of spores by the growing yeast protoplast.Nature. 1959 Apr 18;183(4668):1101-4 PMID: 13657013
-
Production of protoplastlike structures from various species of fungi.Nature. 1962 Nov 17;196:693-4 PMID: 14011321
-
LOCALIZATION OF THE SITE OF FIXATION OF THE INDUCER, PENICILLIN, IN BACILLUS CEREUS.Biochim Biophys Acta. 1964 May 18;87:123-40 PMID: 14167427
-
PRODUCTION OF YEAST AND MOLD PROTOPLASTS BY TREATMENT WITH THE STREPZYME OF MICROMONOSPORA AS.Can J Microbiol. 1965 Jun;11:573-80 PMID: 14346134
-
ENZYMATIC HYDROLYSIS OF YEAST CELL WALLS. I. ISOLATION OF WALL-DECOMPOSING ORGANISMS AND SEPARATION AND PURIFICATION OF LYTIC ENZYMES.J Bacteriol. 1965 Jun;89:1570-80 PMID: 14291597
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