Method for the determination of mean densitometric profiles of chromosomes: application to human chromosomes stained by quinacrine mustard, ethidium bromide or by the Feulgen reaction.

Chromosoma ·Vol. 54 ·No. 1 ·1976-01-27 ·Pages 39-59

Distèche C, Bontemps J

Abstract

When comparing the densitometric profiles of corresponding chromosomes registered from different metaphases or homologous pairs, one is always faced with the variability of their length and overall height. This makes difficult the quantitative comparison of a given chromosome treated by various staining procedures.--A simple and rapid method has been developed for normalizing the densitometric profiles and averaging them in order to obtain a "mean density pattern" of each chromosome. The analysis involves: photographic images, digitalization of the densitometric profiles and processing of the data by a mini-computer.--The method, based on a linear relationship between the area of the densitometric profiles and their length, has been applied to five human chromosomes (1, 2, 6, 12 and 16) stained by ethidium bromide, quinacrine mustard (with or without acidic hydrolysis), pararosaniline and bisaminophenyl-oxadiazole (Feulgen reaction).

MeSH Terms

Bromides Chromosomes/analysis Chromosomes, Human, 1-3 Chromosomes, Human, 16-18 Chromosomes, Human, 6-12 and X DNA/analysis Densitometry/methods Ethidium Humans Hydrolysis Oxadiazoles Quinacrine Mustard Rosaniline Dyes Staining and Labeling

Chemicals

Bromides Oxadiazoles Rosaniline Dyes Quinacrine Mustard DNA Ethidium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Distèche C

Bontemps J

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29 references, click to expand

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Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00331832

Published

1976-01-27

Pages

39-59

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00331832 View journal details

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Method for the determination of mean densitometric profiles of chromosomes: application to human chromosomes stained by quinacrine mustard, ethidium bromide or by the Feulgen reaction.

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