A reliable method of quantifying G-band position in chromosomes.

Chromosoma ·Vol. 50 ·No. 3 ·1975-00-00 ·Pages 301-12

Mainguy PN, Heddle JA, Brunette DM

Abstract

The locations of chromosomal bands in the muntajac (Muntiacus muntjak, Zimmerman) are shown to be constant, that is the bands occupy the same relative position regardless of the state of contraction of the chormosome. Each band can thus be assigned a precise location. Different banding techniques produce bands at identical locations and thus precisely similar patterns, with one notable exception in which certain bands disappear. It it proposed that this more exact procedure be used to identify chromosomal bands, especially in cases of chromosomal rearrangement. It may be of particular use in computer analyses of karyotypes.

MeSH Terms

Animals Chromosome Mapping/methods Chromosomes/analysis Deer Sodium Dodecyl Sulfate Staining and Labeling/methods Statistics as Topic Trypsin Urea

Chemicals

Sodium Dodecyl Sulfate Urea Trypsin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Mainguy P N

Heddle J A

Brunette D M

References (12)

12 references, click to expand

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New technique for distinguishing between human chromosomes.
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Science. 1970 Jun 12;168(3937):1364-6 PMID: 5444269
Staining human metaphase chromosomes with quinacrine mustard before and after standard Giemsa.
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A comparison of prophase and metaphase G-bands in the muntjak.
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Comparison of banding patterns of human chromosomes obtained with heating, fluorescence, and proteolytic digestion.
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Comparison of areas of quinacrine mustard fluorescence and modified Giemsa staining in human metaphase chromosomes.
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Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00283473

Published

1975-00-00

Pages

301-12

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00283473 View journal details

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