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PMID: 4524654 Published · ppublish English Journal Article

Novel properties of satellite DNA from muskmelon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 4 ·1974-04-00 ·Pages 1511-5

Bendich AJ, Anderson RS

Abstract

The purified dense satellite of muskmelon (Cucumis melo L.) represents 30% of the total DNA and forms a sharp, unimodal peak in both neutral and alkaline CsCl gradients. Spectrophotometric melting and DNA reassociation analyses revealed that one-third of the satellite is high melting and has a complexity of about 2.5 x 10(5) daltons, while the remaining two-thirds of the satellite melts 8 degrees lower and has a complexity of about 5 x 10(8) daltons. The thermal stability of reassociated satellite DNA indicates that the multiple copies of the two melting fractions are essentially identical. The sharp, unimodal peak in Cs(2)SO(4) becomes two distinct peaks when either silver or mercuric ions are included in the Cs(2)SO(4) gradient

MeSH Terms
Centrifugation, Density Gradient DNA/analysis Hot Temperature Kinetics Nucleic Acid Denaturation Nucleic Acid Renaturation Seeds/analysis Spectrophotometry
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bendich A J
Anderson R S
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.4.1511
Published
1974-04-00
Pages
1511-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388260
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.4.1511 PMC Full Text View journal details
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