Nucleotide sequence of HS-beta satellite DNA from kangaroo rat Dipodomys ordii.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 9 ·1973-09-00 ·Pages 2642-6

Fry K, Poon R, Whitcome P, Idriss J, Salser W, Mazrimas J, Hatch F

Abstract

The sequence of the highly repetitive satellite HS-beta DNA fraction from kangaroo rat Dipodomys ordii was determined independently by RNA and DNA sequencing techniques. A basic iterated sequence of 10 nucleotides with several mutational variations was found. Base-composition data are consistent with the proposed sequence and revealed a high content of 5-methylcytosine. DNA and RNA sequencing techniques used gave identical results, showing that the fidelity of synthesis of riboguanidine-substituted DNA under our conditions is adequate for nucleotide sequence studies.

MeSH Terms

Adenosine Triphosphate/metabolism Animals Autoradiography Base Sequence DNA/analysis,isolation & purification Electrophoresis Hydrolysis Liver/analysis Mutation Nucleotides/analysis Phosphorus Radioisotopes RNA/biosynthesis Rats/metabolism Ribonucleases Templates, Genetic Uracil Nucleotides/metabolism

Chemicals

Nucleotides Phosphorus Radioisotopes Uracil Nucleotides RNA Adenosine Triphosphate DNA Ribonucleases

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Fry K

Poon R

Whitcome P

Idriss J

Salser W

Mazrimas J

Hatch F

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.9.2642

Published

1973-09-00

Pages

2642-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427073

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.9.2642 PMC Full Text View journal details

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Nucleotide sequence of HS-beta satellite DNA from kangaroo rat Dipodomys ordii.

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