Isolation and chromosomal localization of highly repeated DNA sequences in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 6 ·1971-06-00 ·Pages 1125-9

Botchan M, Kram R, Schmid CW, Hearst JE

Abstract

The nuclear DNA of D. melanogaster contains DNA sequences that are repeated between ten and a hundred times more often than the next class of redundant DNA. This DNA, as a renatured duplex isolated on the basis of its renaturation kinetics, has a buoyant density of 1.691 (g/ml). In its native state it bands within the unique nuclear DNA peak (rho = 1.701). These sequences have been localized by "in situ" hybridization in the chromocenter of the chromosomes of the salivary gland. The properties of centromeres are discussed in terms of the occurrence of repeated sequences at this locus.

MeSH Terms

Animals Autoradiography Base Sequence Centrifugation, Density Gradient Chemistry Techniques, Analytical Chromosomes/analysis DNA/analysis,isolation & purification Drosophila melanogaster Heterochromatin/analysis Hot Temperature Kinetics Models, Structural Molecular Weight Nitrogen Isotopes Nucleic Acid Denaturation Nucleic Acid Hybridization Nucleic Acid Renaturation Salivary Glands/analysis

Chemicals

Heterochromatin Nitrogen Isotopes DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Botchan M

Kram R

Schmid C W

Hearst J E

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.6.1125

Published

1971-06-00

Pages

1125-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389134

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.6.1125 PMC Full Text View journal details

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Isolation and chromosomal localization of highly repeated DNA sequences in Drosophila melanogaster.

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