Relatedness Among Plants as Measured by the DNA-Agar Technique.

Plant physiology ·Vol. 42 ·No. 7 ·1967-07-00 ·Pages 959-67

Bendich AJ, Bolton ET

Abstract

An improved method for extraction of plant DNA is described. Quantitative species comparisons based on DNA-DNA hybridization are reported for several members of the family Leguminosae and for barley, wheat and rye. A maximum of about 10% homology in DNA polynucleotide sequences is found between monocotyledons and dicotyledons tested, whereas 20 to 90% homology is observed within a family. Species compared using a DNA fraction enriched for redundant polynucleotide sequences generally appear to be more closely related than when whole DNA is used. DNA-DNA hybridization may be useful in systematic and evolutionary study of plants, and also as a possible screening procedure for interfertility of species.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bendich A J

Department of Terrestrial Magnetism, Carnegie Institution of Washington, Washington, D. C. 20015.

Bolton E T

References (11)

11 references, click to expand

A MOLECULAR APPROACH IN THE SYSTEMATICS OF HIGHER ORGANISMS. DNA INTERACTIONS PROVIDE A BASIS FOR DETECTING COMMON POLYNUCLEOTIDE SEQUENCES AMONG DIVERSE ORGANISMS.
Science. 1964 May 22;144(3621):959-67 PMID: 14137947
Nucleotide sequence repetition: a rapidly reassociating fraction of mouse DNA.
Science. 1966 Nov 11;154(3750):791-4 PMID: 5919450
A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
An approach to the measurement of genetic relatedness among organisms.
Proc Natl Acad Sci U S A. 1963 Jul;50:156-64 PMID: 13932048
Nuclear DNA and cytoplasmic DNA from tissues of higher plants.
J Cell Biol. 1965 Dec;27(3):451-7 PMID: 5885425
Biochemical correlates of respiratory deficiency. VI. Mitochondrial DNA.
J Mol Biol. 1966 Oct;20(3):453-81 PMID: 5969759
A quantitative assay for DNA-DNA hybrids using membrane filters.
Biochem Biophys Res Commun. 1966 Aug 23;24(4):554-8 PMID: 5338489
Nuclear variation between diploid angiosperms.
Nature. 1966 Aug 20;211(5051):828-30 PMID: 5968749
DNA associated with tobacco chloroplasts.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):207-13 PMID: 5216353
A general method for the isolation of RNA complementary to DNA.
Proc Natl Acad Sci U S A. 1962 Aug;48:1390-7 PMID: 13870855
Thermal renaturation of deoxyribonucleic acids.
J Mol Biol. 1961 Oct;3:585-94 PMID: 14470100

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.42.7.959

Published

1967-07-00

Pages

959-67

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1086656

External Links

PubMed source DOI: 10.1104/pp.42.7.959 PMC Full Text View journal details

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