In vitro methylation of nascent reovirus mRNA by a virion-associated methyl transferase.

Nucleic acids research ·Vol. 1 ·No. 12 ·1974-12-00 ·Pages 1739-52

Faust M, Millward S

Abstract

Chymotrypsin-derived cores, but not virions, catalyze the transfer of methyl groups from S-adenosyl methionine (SAM) to nascent mRNA synthesized in vitro by the core polymerase. The reaction requires Mg(++), and is dependent on the presence of all 4 ribonucleoside triphosphates (rNTPs). Methylation proceeds optimally at 51 degrees C. All ten species of mRNA become methylated during transcription and it is estimated that one methyl residue is incorporated per RNA chain. Experiments designed to determine the location of the methylated nucleotide clearly demonstrate that methylation occurs exclusively at the 5' ends of nascent mRNA.

MeSH Terms

Chymotrypsin Hydrogen-Ion Concentration Kinetics L Cells Magnesium/pharmacology Methyltransferases/metabolism RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Reoviridae/drug effects,enzymology Ribonucleotides S-Adenosylmethionine Time Factors

Chemicals

RNA, Messenger RNA, Viral Ribonucleotides S-Adenosylmethionine Methyltransferases Chymotrypsin Magnesium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Faust M

Millward S

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.12.1739

Published

1974-12-00

Pages

1739-52

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343453

Subset

External Links

PubMed source DOI: 10.1093/nar/1.12.1739 PMC Full Text View journal details

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In vitro methylation of nascent reovirus mRNA by a virion-associated methyl transferase.

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