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PMID: 4338584 Published · ppublish English Journal Article

The 3'-terminal nucleosides of the high molecular weight RNA of avian myeloblastosis virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 5 ·1972-05-00 ·Pages 1176-80

Stephenson ML, Wirthlin LS, Scott JF, Zamecnik PC

Abstract

The RNA isolated from avian myeloblastosis virus was fractionated by sucrose density gradient centrifugation. The 3'-OH terminal nucleosides of various fractions were determined by periodate oxidation followed by tritiated borohydride reduction. The 60-70S fraction and the 35S RNA derived from it by heating both have adenosine as the major terminal nucleoside, with cytidine as the next most frequent terminal. Control samples of tRNA(met) (f. coli) and 28S ribosomal RNA from mouse ascites tumor cells gave the expected terminal residues and molecular weights.

MeSH Terms
Avian Leukosis Virus/analysis Base Sequence Centrifugation, Density Gradient Chemical Phenomena Chemistry Chromatography, Thin Layer Electrophoresis Molecular Weight Nucleosides/analysis Oxidation-Reduction RNA, Viral/analysis,isolation & purification Tritium
Chemicals
Nucleosides RNA, Viral Tritium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stephenson M L
Wirthlin L S
Scott J F
Zamecnik P C
References (46)
46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.5.1176
Published
1972-05-00
Pages
1176-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426657
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.5.1176 PMC Full Text View journal details
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