Characterization of the subunit structure of the ribonucleic acid genome of influenza virus.

Journal of virology ·Vol. 8 ·No. 5 ·1971-11-00 ·Pages 701-7

Lewandowski LJ, Content J, Leppla SH

Abstract

Ribonucleic acid extracted from influenza virus was labeled at the 3' termini with (3)H and analyzed by polyacrylamide gel electrophoresis. Influenza virus was found to contain a minimum of seven and possibly as many as 10 polynucleotide chains, most of which appear to terminate at the 3' end in uridine.

MeSH Terms

Animals Borohydrides Cattle Cell Line Centrifugation, Density Gradient Electrophoresis, Disc Hydrolysis Kidney Molecular Weight Orthomyxoviridae/analysis Oxidation-Reduction Peptide Chain Termination, Translational Peptides/analysis Periodic Acid Phosphorus Isotopes RNA, Viral/analysis,isolation & purification Sucrose Tritium Uridine/analysis Virus Cultivation

Chemicals

Borohydrides Peptides Phosphorus Isotopes RNA, Viral Tritium Periodic Acid Sucrose Uridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lewandowski L J

Content J

Leppla S H

References (19)

19 references, click to expand

Electrophoretic separation of viral nucleic acids on polyacrylamide gels.
J Mol Biol. 1967 Jun 28;26(3):373-87 PMID: 5339793
Electron microscope study of ribonucleic acid of myxoviruses.
J Virol. 1971 Apr;7(4):524-30 PMID: 4106971
Nucleotide sequence studies on yeast phenylalanine sRNA.
Cold Spring Harb Symp Quant Biol. 1966;31:425-34 PMID: 5237199
Polyacrylamide gel electrophoresis of influenza virus RNA.
Virology. 1968 Feb;34(2):385-8 PMID: 5640826
The RNA of influenza virus.
Proc Natl Acad Sci U S A. 1968 Mar;59(3):930-7 PMID: 5238674
Comparison of RNA terminal sequences of phages f2 and Q-beta: chemical and sedimentation equilibrium studies.
Science. 1968 Jun 28;160(3835):1459-60 PMID: 5654059
Studies on the bacteriophage MS2. IV. The 3'-OH terminal undecanucleotide sequence of the viral RNA chain.
J Mol Biol. 1967 Dec 28;30(3):507-27 PMID: 5598208
Terminal sequences of bacteriophage RNAs.
Nature. 1968 Nov 9;220(5167):548-52 PMID: 5686725
Low molecular weight methylated ribonucleic acid species from Chinese hamster ovary cells. I. Isolation and characterization.
Biochemistry. 1969 Mar;8(3):1170-81 PMID: 5781009
Distinct subunits of the ribonucleoprotein of influenza virus.
J Mol Biol. 1969 Jun 28;42(3):485-99 PMID: 5804156
Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity.
Virology. 1969 Sep;39(1):130-4 PMID: 4980034
Structural studies on reovirus: discontinuities in the genome.
Proc Natl Acad Sci U S A. 1970 Feb;65(2):422-9 PMID: 4313198
Polypeptides of avian RNA tumor viruses. II. Serological characterization.
Virology. 1970 Dec;42(4):1113-26 PMID: 4099079
Synthesis and cleavage of influenza virus proteins.
J Virol. 1971 Jan;7(1):155-67 PMID: 5101089
5'-terminus of influenza virus RNA.
Nat New Biol. 1971 Mar 31;230(13):140-2 PMID: 4323758
RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses.
Proc Natl Acad Sci U S A. 1971 Apr;68(4):752-6 PMID: 4325103
Estimations of the molecular weight of the influenza virus genome.
J Gen Virol. 1971 May;11(2):103-9 PMID: 5564417
Characterization of the genome of cytoplasmic polyhedrosis virus.
J Virol. 1971 Apr;7(4):434-7 PMID: 4329560
Acrylamide gel electrophoresis of HeLa cell nucleolar RNA.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1088-95 PMID: 5233837

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.8.5.701-707.1971

Published

1971-11-00

Pages

701-7

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC376250

Subset

External Links

PubMed source DOI: 10.1128/JVI.8.5.701-707.1971 PMC Full Text View journal details

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Characterization of the subunit structure of the ribonucleic acid genome of influenza virus.

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