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PMID: 4257127 Published · ppublish English Journal Article

On the similarity between the tRNAs of organelles and prokaryotes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 2972-6

Fairfield SA, Barnett WE

Abstract

Fluorescence studies with organelle transfer RNAs separated from their cytoplasmic counterparts revealed that phenylalanine tRNA from Euglena chloroplasts or Neurospora mitochondria does not contain a fluorescent "base Y." In contrast, cytoplasmic phenylalanine tRNA from Euglena and cytoplasmic tRNA from Neurospora were found to contain fluorescent bases. The fluorescence-emission spectra of Neurospora cytoplasmic tRNAs and those of the related ascomycete Saccharomyces cerevisiue were observed to be quite different. The results support the generalization that eukaryotic tRNAs contain a fluorescent base, but indicate that their respective organelle tRNAs do not. They also indicate a striking parallelism between organelle and prokaryotic tRNAs.

MeSH Terms
Acylation Chloroplasts/analysis Chromatography Chromatography, DEAE-Cellulose Cytoplasm/analysis Euglena gracilis/analysis,cytology Fluorescence Mitochondria/analysis Neurospora/cytology Neurospora crassa/cytology Phenylalanine/biosynthesis,metabolism Photosynthesis RNA, Transfer/analysis Saccharomyces cerevisiae/cytology Tritium
Chemicals
Tritium Phenylalanine RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fairfield S A
Barnett W E
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.12.2972
Published
1971-12-00
Pages
2972-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389572
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.12.2972 PMC Full Text View journal details
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