Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 3 ·1970-11-00 ·Pages 1207-13

Reger BJ, Fairfield SA, Epler JL, Barnett WE

Abstract

Light-grown, wild type Euglena gracilis contains two aminoacyl-tRNA synthetases for both phenylalanine and isoleucine. Only one of the two synthetases for each amino acid is found in isolated chloroplasts, as are the light-induced phenylalanine and isoleucine tRNAs. In each case the light-induced chloroplast tRNAs can only be acylated by the chloroplast synthetases. The chloroplast isoleucyl-tRNA synthetase is light-inducible and cannot be detected in dark-grown cells or in cells of the bleached mutant W(3)BUL. The presence of the chloroplast phenylalanyl-tRNA synthetase in W(3)BUL, which contains no chloroplast DNA or structure, indicates that this chloroplast enzyme is synthesized in the cytoplasm and is coded by nuclear genes.

MeSH Terms

Amino Acids Chloroplasts/enzymology Chromatography, DEAE-Cellulose Enzyme Induction Euglena Genetic Code Light RNA Nucleotidyltransferases/analysis RNA, Transfer/analysis

Chemicals

Amino Acids RNA, Transfer RNA Nucleotidyltransferases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Reger B J

Fairfield S A

Epler J L

Barnett W E

References (8)

8 references, click to expand

Mitochondrial transfer ribonucleic acids.
Proc Natl Acad Sci U S A. 1967 Feb;57(2):452-8 PMID: 16591491
Differences between mitochondrial and cytoplasmic transfer RNA and aminoacyl transfer RNA synthetases from rat liver.
Proc Natl Acad Sci U S A. 1968 Jul;60(3):1045-52 PMID: 5243921
THE PROTEIN-SYNTHESIZING SYSTEMS FROM THE CYTOPLASM AND THE CHLOROPLASTS OF EUGLENA GRACILIS.
J Mol Biol. 1964 Dec;10:392-402 PMID: 14257693
Mitochondrial-specific aminoacyl-RNA synthetases.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1775-81 PMID: 5231411
A new chromatographic system for increased resolution of transfer ribonucleic acids.
Biochemistry. 1967 Aug;6(8):2507-13 PMID: 4862273
The mitochondrial and cytoplasmic transfer ribonucleic acids of Neurospora crassa.
Biochemistry. 1969 Jun;8(6):2285-90 PMID: 5799126
Induction of euglena transfer RNA's by light.
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1261-8 PMID: 5260929
PROTEIN SYNTHESIS BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. I. AMINO ACID INCORPORATING ACTIVITY OF CHLOROPLASTS IN RELATION TO THEIR STRUCTURE.
Biochemistry. 1965 May;4:865-72 PMID: 14337702

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.3.1207

Published

1970-11-00

Pages

1207-13

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283338

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.3.1207 PMC Full Text View journal details

Analysis Services

Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs.

Contact

WeChat Official Account